亚砷酸钠对HaCaT细胞增殖周期及ROS生成影响  

Effects of sodium arsenic on cell viability,cell cycle and ROS production of human HaCaT cell

作　　者：李昕[1] 刘世宜[1] 袁琴[2] 翁超[2] 李冰[1] 孙贵范[1] 

机构地区：[1]中国医科大学公共卫生学院劳动卫生教研室.辽宁沈阳110001 [2]中国医科大学93期七年制学生

出　　处：《中国公共卫生》2012年第9期1200-1201,共2页Chinese Journal of Public Health

基　　金：辽宁省教育厅科学技术研究基金(L2010705)

摘　　要：目的探讨亚砷酸钠(NaAsO2)对人皮肤角质形成细胞(HaCaT)增殖、细胞周期及活性氧(ROS)生成影响。方法以0、25、50μmol/L NaAsO2作用于HaCaT细胞6 h后,以Alamar Blue还原法检测细胞增殖水平;以流式细胞仪检测细胞周期及ROS水平。结果 25、50μmol/L NaAsO2组细胞增殖水平分别为(93.5±1.18)%、(82.9±2.64)%,明显低于对照组(100±0.51)%(P<0.05);G2/M期细胞数分别为(19.15±0.29)%、(18.12±1.35)%,明显高于对照组(15.24±0.04)%(P<0.05);ROS水平分别为(128.61±6.23)%、(152.92±7.25)%,明显高于对照组(100.00±3.45)%(P<0.05)。结论 NaAsO2作用可引起HaCaT细胞ROS含量增高,同时G2/M期细胞数增多,但G2/M期细胞比例增高并不是由细胞增殖作用所引起。Objective To observe the effects of sodium arsenic on cell proliferation, cell cycle and reactive oxygen species（ROS） production of human HaCaT cell. Methods HaCaT cells were exposed to sodium arsenic at the concentration of 0,25 and 50 p, mol/L for 6 hours. Cells proliferation was measured by Alamar Blue assay, while cell cycle and ROS levels were measured by flow cytometry. Results Cell proliferations of 25 and 50 p, mol/L groups （93.5 % ± 1.18, 82.9% ± 2.64） were significantly lower than that of control,but the ratio of G2/M phase cell（ 19. 15%± 0.29, 18.12% ± 1.35） and ROS levels（ 128.61% ±6. 23,152. 92% ±7.25） of 25 and 50 p, mol/L groups were significantly higher than those of control. Conclusion Sodium arsenic exposure could induce increased ROS production in human HaCaT cells, while the increasing of G2/M cell numbers induced by sodium arsenic exposure does not result from the proliferation of HaCaT cell.

关 键 词：亚砷酸钠(NaAsO2) 细胞增殖 细胞周期 

分 类 号：R994.6[医药卫生—毒理学]