乙酸铅对脑脉络丛Z310细胞毒性作用  

Lead acetate-induced cytotoxicity in Z310 cells

作　　者：王红梅[1,2] 刘羽[1,2] 刘君丽[1,2] 谭壮生[1] 马玲[1,2] 赵超英[1,2] 李国君[1,2] 

机构地区：[1]北京市疾病预防控制中心卫生毒理所,100013 [2]首都医科大学公共卫生与家庭医学学院

出　　处：《中国公共卫生》2012年第9期1202-1204,共3页Chinese Journal of Public Health

摘　　要：目的初步探讨乙酸铅诱导大鼠脑脉络丛Z310细胞的低剂量兴奋作用和高剂量抑制作用。方法以浓度为0、0.000 2、0.002、0.02、0.2、2、20、200、500μmol/L的乙酸铅分别染毒Z310细胞12和24h,用噻唑蓝(MTT)法和2-(2-甲氧基-4-硝苯基)-3-(4-硝苯基)-5-(2,4-二磺基苯)-2H-四唑单钠盐(WST-8)法检测细胞生存情况,并观察各剂量组细胞形态变化。结果 0.02μmol/L乙酸铅染毒24 h,MTT法检测Z310细胞存活率为107.06%,0.2μmol/L染毒组24 h时细胞存活率升高为110.91%;>2μmol/L乙酸铅染毒时,细胞存活率随剂量增加而降低;染毒24 h时,200、500μmol/L乙酸铅组细胞存活率(MTT法)分别下降至79.37%和76.81%(P<0.01);染毒12、24 h时,200、500μmol/L乙酸铅组细胞存活率(WST-8法)分别下降至81.67%和72.36%及56.89%和44.05%(P<0.05)。结论低剂量乙酸铅可引起Z310细胞兴奋效应;高剂量乙酸铅引起Z310细胞抑制效应;WST-8法检测细胞存活率较MTT法更敏感。Objective To detect hormesis effects of lead acetate on Z310 cells. Methods Z310 cells were treated with lead acetate at concentrations of 0,0. 000 2,0. 002,0.02,0.2,2,20,200 and 500 μmol/L for 12 hours and 24 hours. The proliferation viability of Z310 cells was measured by 3-（ 4,5-dimethythiazol-2-yl ） -2,5-diphenyltetrazolium bromide （MTT） and 2- （ 2-methoxy-4-nitrophenyl ） -3- （ 4-nitrophenyl ） -5 （ 2,4-disulfophenyl ） -2H-tetrazolium, monosodium salt （WST-8） assay. And morphological changes in Z310 cells were observed under optical microscope. Results Lead acetate stimulated cell survival rate （107.06%）at lower concentration （0. 02 p, mol /L） for 24 hours. Compared with the control, a significant survival rate increase （ 110. 91%, P 〈 0. 05 ） was observed following exposure to 0. 2 μmol/L lead acetate for 24 hours ,but at higher concentrations（ over 2 μmol/L） ,the survival of the cells was inhibited. The survival rates significantly decreased（79.37% and 76. 81% ） only at 200 and 500 μmol/L lead acetate for 24 hours tested by MTT（P 〈 0. 01 ）. The same effects were observed by WST-8 at the same concentration, with the survival rates of 81.67% and72.36% for 12 hours（P〈0.01） and56.89% and44.05% for24 hours（P〈0.01）.Conclusion Lead acetate can induce the hormesis of Z310 cell proliferation. There is a higher sensitivity in cell survival rate test with WST-8 than MTT.

关 键 词：乙酸铅 Z310细胞 细胞毒性 WST-8法 噻唑蓝(MTT)法 

分 类 号：R994.6[医药卫生—毒理学]