HPLC法同时测定茵胆平肝胶囊中栀子苷、龙胆苦苷、芍药苷的含量  

Simultaneous Determination of Geniposide,Gentiopicrin and Paeoniflorin in Yindan Pinggan Capsules by HPLC

作　　者：陈祯桢 罗珍妹[2] 

机构地区：[1]厦门市妇幼保健院,福建厦门361000 [2]厦门市药品检验所,福建厦门361012

出　　处：《中国药房》2012年第36期3442-3444,共3页China Pharmacy

摘　　要：目的:建立测定茵胆平肝胶囊中栀子苷、龙胆苦苷、芍药苷含量的方法。方法:采用高效液相色谱法。色谱柱为AgilentTC-C18柱,流动相为乙腈-水(16∶84),检测波长为238nm,进样量为5μL,流速为1.0mL·min-1,柱温为30℃。结果:栀子苷进样量在0.0826～1.652μg范围内、龙胆苦苷进样量在0.1290～2.580μg范围内、芍药苷进样量在0.0446～0.892μg范围内与各自峰面积积分值均呈良好的线性关系;栀子苷、龙胆苦苷、芍药苷的平均回收率分别为99.8%、100.0%、100.1%,RSD分别为1.89%、1.84%、1.82%(n=9)。结论:本法操作简便、结果准确、灵敏度高、重复性好,可用于茵胆平肝胶囊的质量控制。OBJECTIVE： To establish a method for the simultaneous determination of geniposide, gentiopicrin and paeoniflorin in Yindan pinggan capsules. METHODS： HPLC method was adopted. The separation was performed on Agilent TC-C18 column with mobile phase consisted of acetonitrile-water （16：84） at the flow rate of 1.0 mL.min^-1. The detection wavelength was set at 238 nm, and injection volume was 5 ixL. The temperature of column was 30℃. RESULTS： The linear range was 0.082 6-1.652μg for geniposide, 0.129~2.580 ~tg for gentiopicrin and 0.044 6-0.892μtg for paeoniflorin, and the average recoveries of them were 99.8 % （RSD= 1.89%, n=9）, 100.0% （RSD= 1.84%, n=9）, 100.1% （RSD= 1.82%, n=9）, respectively. CONCLUSION ： The method is simple, accurate, sensitive and reproducible, which can be used for the quality control of Yindan pinggan capsules.

关 键 词：茵胆平肝胶囊 栀子苷 龙胆苦苷 芍药苷 高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学]