七氟烷对新生大鼠学习记忆功能的影响  

Effects of sevoflurane on the function of learning and memory in neonatal rats

作　　者：徐桂萍[1] 张永杰[1] 唐冬梅[1] 

机构地区：[1]新疆维吾尔自治区人民医院麻醉科,乌鲁木齐830000

出　　处：《国际麻醉学与复苏杂志》2012年第9期584-586,592,共4页International Journal of Anesthesiology and Resuscitation

基　　金：新疆维吾尔自治区自然科学基金资助项目（2011211A056）

摘　　要：目的探讨七氟烷麻醉对新生大鼠学习记忆功能的影响。方法新生7dSD大鼠80只，采用完全随机设计随机分为5组（每组16只）：A、B组分别吸入3％七氟烷6h和2h；C、D组分别吸入1．5％七氟烷6h和2h；E组吸入空气对照。新生大鼠麻醉后分别于第21—23天（I期）和第60～62天（Ⅱ期）各组行Y型迷宫试验后立即处死，取大鼠脑海马组织，进行免疫组织化学染色，测定N．甲基-D-天冬氨酸受体1（N-methy-D-aspatratereceptor1，NMDARl）的表达。结果与E组比较，A组I期Y型迷宫第1天测试结果中的全天总反应时间（totalreactiontime，TRT）和错误次数（errornumber,EN）分别为（100±19）S和（9．2±1．1）次（P〈O．05），第2天～第3天时无统计学差异，余各组I期Y型迷宫测试结果无统计学差异（D0．05）；与E组比较，Ⅱ期各组Y型迷宫测试结果均无统计学差异（P〉0．05）。I、Ⅱ期各组海马NMDARl的表达差异均无统计学意义（p〉0．05）。结论吸入1．5％七氟烷不会导致幼年大鼠学习记忆功能减退；吸人3％七氟烷6h对幼年大鼠早期可产生一过性学习记忆功能减退，对远期学习记忆功能未见明显影响。Objective To investigate the effects of sevoflurane inhalation anesthesia on learning and memory function in neonatal rats. Methods Eighty 1 week old SD rats were randomly divided into 5 groups with 16 rats each group： A, B groups of inhaled 3% sevoflurane 6 h and 2 h;, C,D group inhaled 1.5% sevoflurane 6 h and 2 h; group E inhaled air as control. Learning and memory function was assessed using Y-maze test at P21 -P23 （ I stage） and P60-P62 （ Ⅱ stage） after anesthesia. The animals were killed immediately after the tests and hippocampus was isolated and expression using immuno-histoehemical technique. Results The first day of total reaction time（TRT） and error number（EN） in group A I stage Y-maze were （100±19） s and （9.2±1.1） times compared with control group E （P〈0.05）, there was no difference in 2th-3th day, the other groups showed no difference （P〉0.05）; there were no differences in Ⅱ period Y-maze test （P〉0.05）. While there was no significant difference in N-methy-D-aspatrate receptor 1 （NMDAR1） expression of each group in I and Ⅱ stage （P〉0.05）. Conclusions Inhalation of 1.5% sevoflurane does not induce learning and memory function decline in neonatal rats. Inhalation of 3.0% sevoflurane 6 h can induce transient learning and memory functiondecline in neonatal rats, and there was no significant difference for future learning and momory.

关 键 词：七氟烷 海马 学习记忆 N-甲基-D-天冬氨酸受体 

分 类 号：R614[医药卫生—麻醉学]