TLR2胞外段特异性抗体对TLR2激动剂诱导的炎症和促过敏反应的抑制作用  

The inhibition of antibodies against mouse TLR2 extracellular domain on the inflammation and allergic response induced by agonists of TLR2

作　　者：潘庆军[1,2] 刘渊[1] 朱平[1] 侯晓睿[1] 刘艳君[1] 

机构地区：[1]南方医科大学基础医学院免疫学教研室,广州510515 [2]广东医学院肾脏疾病研究所,湛江524001

出　　处：《中国免疫学杂志》2012年第8期685-689,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金项目资助(No.30671970)

摘　　要：目的:观察小鼠TLR2胞外段抗原肽特异性抗体对TLR2激动剂诱导的炎症和促过敏反应的影响。方法:用小鼠TLR2胞外段单表位抗原肽(T20)免疫动物制备抗体(T20抗体);体外观察T20抗体对PGN和LTA刺激RAW264.7细胞生成TNF-α和IL-6的影响,用ELISA法检测各组TNF-α和IL-6量;体内观察T20抗体对OVA致敏小鼠PGN致死性攻击的影响,记录各组直肠温度变化和死亡率。结果:获得小鼠TLR2特异性T20抗体;该抗体可抑制TLR2激动剂(PGN和LTA)对相应靶细胞的刺激作用,使其生成的TNF-α和IL-6显著降低;体内实验证实T20抗体对PGN加剧的过敏反应具有抑制作用。结论:T20抗体可特异结合小鼠TLR2,抑制TLR2激动剂诱导的炎症及TLR2激动剂加剧的过敏反应。Objective：To study the inhibitory effects of antibodies against mouse TLR2 extracellular domain on the inflammation and allergic response induced by agonists of TLR2.Methods：The specific antibodies（anti-T20） of 20mer peptide epitope of mouse TLR2 extracellular domain（T20） were prepared by immunized T20 in rabbits.Effects of anti-T20 on PGN and LTA-driven TNF-α and IL-6 production in vitro were studied and the production of TNF-α and IL-6 were tested by ELISA.Also,the effect of anti-T20 on PGN-driven death of OVA allergic mice in vitro was studied,and rectal temperature and mortality were recorded.Results：Anti-T20 could specifically bind with mouse TLR2 and significantly inhibit PGN and LTA-driven TNF-α and IL-6 production by RAW264.7 cells.Also,anti-T20 could partially protect OVA allergic mice from PGN-induced death.Conclusion：Anti-T20 can specifically bind to mouse TLR2 and inhibit TLR2 agonist-driven inflammation and TLR2 agonist-promoting allergic response.

关 键 词：TLR2 胞外段表位 抗体 炎症 过敏 

分 类 号：R392.12[医药卫生—免疫学]