大鼠羊水栓塞后类胰蛋白酶、TNF-α水平变化的研究  

The change of trptase、TNF-α in amniotic fluid embolism after laboring in rats

作　　者：王茜[1] 袁晓洁[1] 高德红[1] 王丽萍[1] 郝玉凤[1] 

机构地区：[1]齐齐哈尔医学院第五附属龙南医院,163453

出　　处：《中国优生与遗传杂志》2012年第8期37-39,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的探讨类胰蛋白酶、TNF-α在羊水栓塞中的变化及病理生理作用。方法精选雌性鼠30只于妊娠20天制作羊水栓塞模型。根据注入液体性质不同随机分为对照组(10只)、羊水组(10只)、胎粪液组(10只)。对照组除注入生理盐水外,皆同其余组。实验后取鼠左肺行H.E染色光镜下观察,采用专性底物对模型鼠的血清类胰蛋白酶酶活力进行测定。免疫法检测血中肿瘤坏死因子(TNF-α)含量。结果 1.实验组肺组织可见大量炎性细胞浸润,包括白细胞(主要为中性粒细胞)、巨噬细胞及少量淋巴细胞。2.生理盐水组注入前后类胰蛋白酶水平无明显差异(P>0.05);鼠原羊水组和胎粪液组注入后类胰蛋白酶水平明显升高,差异显著(P<0.01)。3、实验组中TNF-α含量较对照组高,差异显著(P<0.01)。结论肥大细胞脱颗粒释放类胰蛋白酶可能是羊水栓塞的重要原因,在此基础上引起中性粒细胞、巨噬细胞浸润及一系列病理变化,由此引起肺损伤及临床症状。该酶学方法灵敏、有效,在羊水栓塞临床早期诊断中有一定的应用价值。Objective： To evaluate the change and role of trptase , TNF-α in amniotic fluid embolism after laboring in rats. Methods： Strictly selected 30 Wistar rats （late pregnancy） and randomly divided into the Control group, amniotie group, meeonium group. The procedure for control group was exactly the same as the experimental group except injecting NS. After the experiment, the left lung of the rat was perfused with 10% Formalin, H. E, the enzyme activity was tested colormetrically using special substrate, BAPNA. Immunoassay detecition of tumor necrosis factor （ TNF-α） levels. Results ： 1. There are lots of infiltration of neutrophil and macrophage in pulmonary tissue of experimental group. 2. There are no change of trptase levels in control group （ P 〉 0.05 ） ; All experimental group has abnormally high levels of Trptase compared with periinfusion values （ P 〈 0.01 ）. 3, Experimental group, TNF-α levels than the control group significant difference （P 〈0. 01 ）. Conclusion： Rat amniotie fluid may induce degranulation of mast cells to release trptase, These results suggest a role for mast cell activation in the mechanism of amniotic fluid embolism. The method is sensitive and efficiency, it is worth to diagnose amniotic fluid embolism.

关 键 词：羊水栓塞 肺损伤 类胰蛋白酶 肿瘤坏死因子 

分 类 号：R714.46[医药卫生—妇产科学]