肠道病毒基因分型检测研究进展  

Advance in genotyping detection on enterovirus

作　　者：王宇平[1] 鄢雪梨 

机构地区：[1]福建国际旅行卫生保健中心,福建福州350001 [2]福建卫生职业技术学院

出　　处：《中国国境卫生检疫杂志》2012年第4期286-288,共3页Chinese Journal of Frontier Health and Quarantine

基　　金：福建出入境检验检疫局科研基金项目(FK2008-10)

摘　　要：采用传统细胞培养、中和试验对肠道病毒进行分型费时、费力,而分子生物学技术,包括限制性内切酶片断长度多态性(Restriction Fragment Length Polymorphism,RFLP)、核酸杂交、基因序列分析、多重PCR、基因芯片等,更加快速、敏感、特异,越来越多应用于肠道病毒的分型检测。本文对肠道病毒的型别、基因分型依据及分子生物学分型检测方法进行综述。The conventional typing of EV strains consists of isolating virus in cell culture, followed by neutralization test, which were time consuming and labor intensive. Molecular techniques, including restriction fragment length polymorphism, hybridization, nucleotide sequence analysis, multiplex, gene chip, are more rapid, sensitive and specific, which is widely used in classified of EV. This paper reviewed EV types, and the progress of the basis and molecular biology method for EV genotyping.

关 键 词：肠道病毒 检测 基因分型 

分 类 号：R512.5[医药卫生—内科学]