青岛百合包埋-玻璃化法超低温保存技术研究  

Study on Cryopreservation of Lilium tsingtauense GilgLiliaCeae by Encapsulation-Vitrification Method

作　　者：王金录[1] 钤泰琳[1] 赵美爱[1] 

机构地区：[1]青岛农业大学生命科学学院,青岛市主要农作物创新与应用重点实验室,山东青岛266109

出　　处：《北方园艺》2012年第18期135-139,共5页Northern Horticulture

基　　金：山东省中青年科学家科研奖励基金资助项目(2007BS06011);青岛农业大学博士启动基金资助项目(630621)

摘　　要：以青岛百合茎尖为试材,研究了外植体的选择、预培养的蔗糖浓度、预培养时间、包埋预处理方法、PVS2处理时间对百合存活率的影响。结果表明:小鳞茎在4℃冷锻炼培养28d后,剥取2～3mm的茎尖为试验材料,在蔗糖浓度0.4mol/L的MS+100g/L海藻糖+1mg/LABA的培养基上,预培养3d,然后加0.4mol/L蔗糖和2mol/L甘油、3%褐藻酸钠进行包埋预处理,在0℃条件下用PVS2处理60min,投入液氮保存1h,38℃解冻2min、洗涤后转接至恢复培养基上暗培养7d,用TTC检测存活率最高可达到67.5%。Taking shoot tips（2-3 nun） of Liliurn tsingtauense GilgLiliaCeae. from 28 days cold-hardened bulblets at 4°C as materials, effects of different explants, sucrose concentration of pre-culture, duration of pre-culture, different encapsulation pretreatment,duration of treatment with PVS2 were studied. The results showed that the most appropriate sucrose concentration was 0.4 mol/L during pre-incubation and the best pre-eulture time was 3 d. Shoot-tips wereencapsulated in 3% sodium alginate including 0. 4 mol/L sucrose and 2 mol/L glycerol after pre-incubation,and PVS2 for 60 min at 0°C ,plunged directly into liquid nitrogen for 1 h,then thawed for 2 min at 38°Cand washed. At last,the shoot tips were cultured on recovery medium. The highest survival rate was 67. 5 % with TTC staining method.

关 键 词：青岛百合 茎尖 包埋-玻璃化法 超低温保存 

分 类 号：S682.29[农业科学—观赏园艺]