拒盐型牧草小花碱茅PutHKT2;1基因表达模式分析  

Expression pattern analysis of a high-affinity K+ transporter gene PutHKT2;1 from halophyte Puccinellia tenuiflora

作　　者：李剑[1] 张金林[1] 

机构地区：[1]兰州大学草地农业科技学院草地农业生态系统国家重点实验室,甘肃兰州730020

出　　处：《草业科学》2012年第9期1379-1383,共5页Pratacultural Science

基　　金：国家自然科学基金项目(31172256);教育部"新世纪优秀人才支持计划"项目(NCET-11-0217);兰州市科技发展计划项目(2010-1-39);中央高校基本科研业务费专项资金项目(lzujbky-2010-2)

摘　　要：本研究以拒盐型盐生牧草小花碱茅(Puccinellia tenuiflora)为材料,采用RT-PCR方法对小花碱茅PutH-KT2;1基因表达模式进行研究,以期阐明其在盐胁迫下拒Na+的分子机制。结果表明,PutHKT2;1基因表达存在组织特异性,主要在根中表达,地上部表达量相对较低;K+饥饿处理条件下,在根中的表达量最高;在1mmol.L-1 K+或无K+条件下,根中PutHKT2;1表达量随着盐浓度的增大而减少。由此推断,PutHKT2;1蛋白主要在根部发挥作用,主要用于控制Na+吸收,增强K+选择性吸收能力,从而维持地上部较高的K+/Na+,提高其耐盐性。The HKT transporters from higher plants function as relatively K＋ --Na＋ transporters, playing vital roles in salt tolerance. We analyzed the expression pattern of PutHKT2 ; 1 from Puccinellia tenuiflo- ra by RT-PCR method. The results showed that the expression of PutHKT2 ： 1 was induced dramatically in roots under K＋-starvation stress for 24 h, but its expression was only slightly regulated by all stresses in shoots, therefore, the expression of PutHKT2;1 had tissue specificity. Under 1 mmol · L-1 K＋ or K＋- starvation stress, PutHKT2;I expression gradually decreased with salt concentration increase. PutH- KT2;1 may play an important role in roots, which may control Na＋ uptake, strengthen the selectivity of K＋ over Na＋ and enhance salt tolerance.

关 键 词：小花碱茅 PutHKT2 1 表达模式分析 耐盐性 

分 类 号：S543.9[农业科学—作物学]