黄芩种质资源的ISSR分析  

Studies on germplasm resources of Scutellaria baicalensis using ISSR markers

作　　者：张红瑞[1] 马彦秋[1] 高致明[1] 王文全[2] 

机构地区：[1]河南农业大学中药材工程技术研究中心,河南郑州450002 [2]北京中医药大学中药学院,北京100102

出　　处：《河南农业大学学报》2012年第4期380-384,共5页Journal of Henan Agricultural University

基　　金：河南省教育厅自然科学研究计划项目(2011B360003)

摘　　要：采用ISSR分子标记,对野生黄芩(Scutellaria baicalensis)54个种源基因组DNA进行PCR扩增.利用SPSS11.5软件对种源间亲缘关系进行分析,构建遗传性状图.结果表明,从100条ISSR引物中筛选出扩增条带多、清晰和稳定性好的9条引物,在黄芩54个种源中共得到2 279条带,1 577条为多态性条带,多态性位点68.73%,聚类分析将黄芩54个种源分为3个分支,表明野生黄芩种源间遗传变异丰富.PCR amplification was performed with the genomic DNA of fifty-four kinds of wild Scutellaria baicalensis from different germplasms by ISSR molecular markers. The genetic relationships were analyzed by software SPSS 11.5, and the DNA molecular dendrogram was established. The results showed that nine polymorphic primers for identification were chosen from 100 ISSR primers. A total of 2 279 bands were amplified from fifty-four kinds of Scutellaria baicalensis using the nine screened primers, including 1 577 polymorphie bands taking up about 68.73%. A systematic cluster analysis shows that the fifty-four kinds of wild Scutellaria baicalensis can be divided into three groups, sugges- ting that there are abundant genetic diversities among the fifty-four kinds of wild Scutellaria baicalensis from germplasms.

关 键 词：黄芩 种质资源 简单重复序列间扩增 遗传多样性 

分 类 号：S567[农业科学—中草药栽培]