藿香正气口服液中3个活性成分的UPLC法测定  

Determination of Three Active Ingredients in Huoxiang Zhengqi Oral Liquid by UPLC

作　　者：邓开英[1,2] 余佳文[3] 彭涛[3] 夏永鹏 邱宗荫[1] 

机构地区：[1]重庆医科大学,重庆400016 [2]重庆市食品药品检验所,重庆401121 [3]太极集团重庆涪陵制药厂有限公司,重庆408000 [4]重庆市卫生局,重庆401147

出　　处：《中国医药工业杂志》2012年第9期780-782,共3页Chinese Journal of Pharmaceuticals

摘　　要：建立了超高效液相色谱法同时测定藿香正气口服液中的橙皮苷、和厚朴酚及厚朴酚。采用C18色谱柱,以乙腈-0.05%磷酸为流动相,梯度洗脱,检测波长为220 nm。橙皮苷、和厚朴酚与厚朴酚分别在6.4～128 ng、3.2～64 ng和6.4～128 ng范围内线性关系良好,平均回收率分别为98.3%、98.4%和102.2%,RSD分别为0.5%、0.9%和2.0%。10批样品测定结果与中国药典HPLC方法测定结果一致。An UPLC method was established for the determination of hesperidin, honokiol and magnolol in Huoxiang Zhengqi oral liquid. A C18 column was used with the mobile phase of acetonitrile-0.05 % phosphoric acid by gradient elution at the detection wavelength of 220 nm. The calibration curves were linear in the ranges of 6.4 - 128 ng, 3.2 - 64 ng and 6.4 - 128 ng, respectively. The average recoveries were 98.3 %, 98.4 % and 102.2 %, with RSDs of 0.5 %, 0.9%0 and 2.0%, respectively. The results for 10 batches of sample were consistent with those by HPLC in Chinese Pharmacopoeia.

关 键 词：藿香正气口服液 橙皮苷 和厚朴酚 厚朴酚 超高效液相色谱 测定 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.72[化学工程—制药化工]