舒肝袪脂胶囊中羟基红花黄色素A和大黄素的HPLC法测定  

Determination of Hydroxysafflor Yellow A and Emodin in Shuganquzhi Capsules by HPLC

作　　者：刘庆丰[1] 施孝金[1] 李中东[1] 王晓萍[2] 钟明康[1] 

机构地区：[1]复旦大学附属华山医院临床药学室,上海200040 [2]复旦大学附属华山医院口腔科,上海200040

出　　处：《中国医药工业杂志》2012年第9期782-784,共3页Chinese Journal of Pharmaceuticals

摘　　要：建立了高效液相色谱法同时测定舒肝袪脂胶囊中的羟基红花黄色素A和大黄素。采用C18色谱柱,以甲醇-1%乙酸为流动相,梯度洗脱,检测波长254 nm。羟基红花黄色素A和大黄素在0.1～60 g/ml和0.1～6 g/ml浓度范围内线性关系良好,平均回收率大于98%,日内和日间RSD均小于2.0%。An HPLC method was established for the simultaneous determination of hydroxysafflor yellow A and emodin in Shuganquzhi capsules. A C18 column was used with the mobile phase of methanol -1% acetic acid by gradient elution at the detection wavelength of 254 nm. The calibration curves of hydroxysafflor yellow A and emodin were linear in the concentration ranges of 0.1 - 60 μg/ml and 0.1 - 6 μg/ml, respectively. The recoveries were over 98 %, with intra- and inter-day RSDs less than 2.0%.

关 键 词：舒肝祛脂胶囊 羟基红花黄色素A 大黄素 高效液相色谱 测定 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.72[化学工程—制药化工]