利用CEL Ⅰ酶鉴定水稻广亲和基因S5位点的籼粳属性  

Molecular Classification of indica-japonica Rice According to Wide-compatibility Gene S5 Sequence with Endonuclease CEL Ⅰ

作　　者：倪深[1] 肖文斐[2] 陈红旗[1] 王跃星[1] 朱旭东[1] 

机构地区：[1]中国水稻研究所水稻生物学国家重点实验室,浙江杭州310006 [2]杭州市农业科学研究院生物技术研究所,浙江杭州310024

出　　处：《中国水稻科学》2012年第5期549-554,共6页Chinese Journal of Rice Science

基　　金：转基因生物新品种培育重大专项(2011ZX08001-004)

摘　　要：广亲和基因S5的克隆为水稻品种亲和特性的鉴定提供了分子学依据。利用PCR技术对水稻S5位点中籼、粳序列间存在差异的区段进行扩增,并结合芹菜核酸内切酶CELⅠ对其进行酶切鉴定,从而区分S5不同的基因型。研究结果表明该方法能准确鉴定不同水稻品种中S5位点的籼粳特性,并能发现新的差异序列。Cloning of the major locus S5, a wide compatibility gene has laid a molecular basis for identification of the wide-compatibility variclies（WCV）. According to the difference in S5 locus sequence among indica, japonica and WCV, PCR was conducted and celery endonuclease CEL Ⅰ , which cleaves DNA with high specificity at sites of basesubstitution mismatch, was used to digest PCR products so as to distinguish various S5 DNA fragments. The results showed that the method is accurate in indica japonica classification. At the same time, a new genotype on the S5 locus was found.

关 键 词：水稻 广亲和 S5基因 籼粳属性 

分 类 号：S511[农业科学—作物学]