新疆野苹果分离群体的构建和评价  

Construction and evaluation of the segregation population in Malus sieversii

作　　者：刘遵春[1,2] 苗卫东[2] 刘大亮[1] 陈学森[1] 

机构地区：[1]山东农业大学园艺科学与工程学院/作物生物学国家重点实验室,山东泰安271018 [2]河南科技学院园艺园林学院,河南新乡453003

出　　处：《果树学报》2012年第5期722-728,共7页Journal of Fruit Science

基　　金：国家自然科学基金项目(31171932);山东省农业良种工程项目(鲁农良种字[2011]7号)

摘　　要：【目的】为了建立合适的用于构建新疆野苹果遗传连锁图镨的分离群体,【方法】以新疆红肉苹果和‘红富士’为材料,利用人工授粉方法构建了分离群体,采用SSR分子标记技术对作图亲本多态性、杂种纯度和群体内部的遗传结构进行了检测。【结果】结果表明,新疆红肉苹果和‘红富士’的遗传差异较大;排除了15株不确定的个体,确定110株作为新疆野苹果遗传图谱构建的作图群体;株系间无重复现象;64对SSR引物组合在作图亲本间共检测到232个多态性位点,其中有191个位点在P<0.01水平上符合孟德尔期望分离比,占多态性位点总数的82.30%。【结论】选取该群体作为构建新疆野苹果遗传连锁图镨的分离群体是合适的。[Objective]The objective of the study is to construct the optimal segregation population for constructing the genetic linkage map in Malus sieversii. [Method]Using red ‘Fuji＇ ×Xinjianghongrou apple as experimental material, a segregation population for M. sieversii had been established by artificial pollination, and the polymorphism between parents, hybrid purity and genetic structure among all progenies had been detected by microsatellite marker. [Result]The results indicated that there was larger genetic variation between red＇Fuji＇ and Xinjianghongrou apple. Excluding 15 undefined progenies, 110 progenies of the segregation population were used to construct the genetic linkage map in M. sieversii. There was no repetition phenomenon among all progenies. 232 polymorphic fragments were detected by 64 SSR primers, among which 191 （82.3%） segregated in the expected Mendelian 1：1 ratio （P〈 0.01）. [Conclusion] The studies suggested that the segregation population of red ‘Fuji＇ ×Xinjianghongrou apple was optimal to construct the genetic linkage map in M. sieversii.

关 键 词：新疆野苹果 SSR 分离群体 

分 类 号：S661.1[农业科学—果树学]