大豆NF-YB家族全基因组鉴定、分类和表达  

Genome-Wide Identification,Classification,and Expression of NF-YB Gene Family in Soybean

作　　者：郑炜君[1,2] 徐兆师[2] 冯志娟[2] 李连城[2] 陈明[2] 柴守诚[1] 马有志[2] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100 [2]中国农业科学院作物科学研究所,农作物基因资源与基因改良国家重大科学工程/农业部作物遗传育种重点开放实验室,北京100081

出　　处：《作物学报》2012年第9期1570-1582,共13页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31171546);国家转基因生物新品种培育科技重大专项(2011ZX08002-002,2009ZX08009-083B)资助

摘　　要：利用大豆基因组数据库,通过生物信息学手段,鉴定大豆NF-YB家族基因的全序列、定位和拷贝数,通过序列比对进行进化和分类分析。利用大豆高通量RNA测序数据、NCBI中UniGene的EST表达数据进行组织表达谱分析。结果表明,大豆基因组中含有28个NF-YB家族基因,分布于大豆的14条染色体上,系统进化树分析将其分成3类。启动子分析表明,几乎全部NF-YB家族基因的启动子区均含有逆境应答反应顺式作用元件。各个发育阶段中,多数成员至少在一个组织中表达,10个差异表达的基因中有2个在根瘤中特异表达,2个在根中特异表达,2个在根瘤中优势表达,另外4个在其他部位优势表达。研究结果促进了NF-YB家族基因的功能研究与利用。Based on soybean genome database and bioinformatics method, we obtained soybean NF-YB family genes and their positions on chromosome and duplication information. NF-YB proteins were classified according to their phylogenetic relation- ship. The soybean RNA-sequencing data from the SoyBase database and EST data from NCBI UniGene were used to analyze the expression pattern of these genes at different development stages. A total of 28 NF-YB genes were systematically identified from soybean and classified into three types. They were located on 14 chromosomes and most of them had stress related cis-acting ele- ments in their promoter regions. Ten differentially expressed genes were found at each developmental stage, and four of them were highly expressed in different tissues other than root nodules and root. Among other six genes, two were highly expressed in root nodule, two were specifically expressed in root nodules and two were specifically expressed in root. The results facilitate functional analysis and utilization of NF-YB genes in crop genetic improvement.

关 键 词：NF—YB家族 全基因组鉴定 进化 差异表达 启动子 

分 类 号：S565.1[农业科学—作物学]