薰衣草精油对大鼠下丘脑及杏仁核c-fos表达的影响  

Effects of lavender essential oil on the expression of c-fos in the hypothalamus and amygdala of rats

作　　者：操礼琼[1] 杨莹[2] 程文文[1] 李光武[3] 傅佳[1] 

机构地区：[1]安徽医科大学第一附属医院,合肥230022 [2]安徽医科大学第四附属医院,合肥230000 [3]安徽医科大学神经生物学研究所,合肥230032

出　　处：《中华中医药杂志》2012年第9期2432-2434,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：安徽省自然科学基金(No.090413145)~~

摘　　要：目的:观察薰衣草精油对大鼠下丘脑、杏仁核即早基因(c-fos)表达的影响,探讨其发挥生物活性的中枢机制。方法:将24只SD大鼠随机分为薰衣草精油组、蒸馏水组和空白组,免疫组化法观察各组大鼠下丘脑、杏仁核c-fos表达并对结果进行灰度半定量分析。结果:c-fos阳性细胞呈棕黄色染色,薰衣草组大鼠c-fos阳性细胞主要分布在杏仁核、下丘脑处,其它脑组织有散在分布,蒸馏水组和空白组大鼠c-fos阳性细胞散在分布于各处脑组织。与蒸馏水组和空白组比较,薰衣草组下丘脑、杏仁核c-fos灰度值降低(P<0.05)。结论:大鼠吸入薰衣草精油后可能通过下丘脑及杏仁核发挥各种生物活性。Objective： To study the effects of lavender essential oil on the expression of c-fos in the hypothalamus and amygdala of rats, explore the biological activity of the central mechanisms to lavender essential oil. Methods： Twenty-four SD rats were randomly divided into three groups： lavender essential oil group, distilled water group and control group. The immuno histochemical methods were used to detect the c-fos expression in the hypothalamus, amygdala and was semi-quantitative analysed for grey value. Results： The c-fos positive neurons were dyed in brown. For lavender essential oil group, c-fos positive neurons were mainly distributed in the hypothalamus and amygdala; others brain were scattered. For distilled water and control group, c-fos positive neurons were dispersed in brain tissue. Compared to distilled water and control group, grey value of c-fos of lavender was significantly reduced （P〈0.05） in the hypothalamus and amygdala. Conclusion： Rats after inhalation of lavender essential oil may play a variety of biological activity through the hypothalamus and amygdala.

关 键 词：薰衣草精油 下丘脑 杏仁核 C-FOS基因 

分 类 号：R285.5[医药卫生—中药学]