多波长HPLC法同时测定栀子药材中3种有效成分的含量  

Simultaneous determination of three bioactive components in Gardeniae Fructus by HPLC under multiple UV wavelengths

作　　者：刘若楠[1] 杨志玲[1] 莫润宏[1,2] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,富阳311400 [2]国家林业局经济林产品质检中心(杭州)质检部,富阳311400

出　　处：《中华中医药杂志》2012年第9期2438-2440,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家科技部公益性林业专项资助项目(No.200704022)~~

摘　　要：目的:利用多波长高效液相色谱法,建立同时测定栀子药材中3种有效成分(栀子苷、绿原酸和西红花苷-Ⅰ)含量的检测方法。方法:采用Waters XBrige C18色谱柱(250mm×4.6mm,5μm),柱温30℃;以乙腈-0.4%磷酸水溶液为流动相梯度洗脱,流速1.0mL/min;检测波长238nm(检测栀子苷)、327nm(检测绿原酸)和440nm(检测西红花苷-Ⅰ)。结果:在30min内栀子中栀子苷、绿原酸和西红花苷-Ⅰ3种有效成分被完全分离;线性范围分别为0.034-0.204mg/mL(r=0.9999)、0.014-0.082mg/mL(r=0.9998)、0.024-0.142mg/mL(r=0.9999);平均加样回收率分别为101.88%(RSD为0.87%)、99.23%(RSD为1.47%)、98.91%(RSD为1.15%)。结论:本方法快速简便、重复性好,能够为更好的控制栀子药材的质量提供科学依据。Objective： To develop an HPLC method with multiple UV wavelengths detection for the simultaneous determination of Geniposide, Chlorogenic Acid and Crocin-I in Gardeniae Fructus. Methods： The samples were separated on a Waters XBrige C18 column （250mmx4.6mm, 50m）, by a gradient elution using acetonitrile and 0.4% phosphoric acid as mobile phase at a flow rate of 1.0mL/min. The eluate was detected by multiple wavelengths 238nm for Geniposide, 327nm for Chlorogenic Acid, and 440nm for Crocin-I. Results： The complete separation was obtained within 30 minutes for the three compounds （Geniposide, Chlorogenic Acid and Crocin-I）. The linear ranges of them were 0.034-0.204 mg/mL （r=0.9999）, 0.014-0.082mg/mL （r=-0.9998） and 0.024-0.142 mg/mL （r=0.9999）, with the average recoveries （n=5） were 101.88% （RSD=0.87%）, 99.23% （RSD=l.47%） and 98.91% （RSD=1.15%） respectively. Conclusion： The assay demonstrated that the method is simple, rapid and reproducible for the quality control of Gardeniae Fructus.

关 键 词：栀子 高效液相色谱法 栀子苷 绿原酸 西红花苷-Ⅰ 

分 类 号：R284.1[医药卫生—中药学]