加味藿朴夏苓汤对温病湿热证大鼠舌组织AQP-5的影响  

Effect of modified Huopu Xialing Decoction on AQP-5 expression of tongue tissue in rat with epidemic febrile disease of damp-heat syndrome

作　　者：常丽萍[1] 阙铁生[1] 吕军影[1] 李曙光[1] 王华[1] 黄李平[1] 何雪萍[1] 

机构地区：[1]广西医科大学第一附属医院,南宁530021

出　　处：《中华中医药杂志》2012年第9期2458-2461,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：广西自然科学基金项目(No.2010GXNSFA013146);广西研究生教育创新计划课题(No.2009105981006M225);广西壮族自治区卫生厅重点科研课题(No.重2010023);广西壮族自治区卫生厅立项课题(No.z2010385)~~

摘　　要：目的:观察加味藿朴夏苓汤干预对温病湿热证模型大鼠舌组织水通道蛋白-5(AQP-5)的影响。方法:24只SD大鼠,随机分为正常对照组、湿热模型组、药物干预组。参照多因素复合造模方法复制湿热证大鼠模型。光镜下观察舌组织病理改变,采用免疫组化方法检测AQP-5的表达情况。结果:湿热模型大鼠有腻苔形成,药物干预组腻苔出现率较湿热模型组明显减少(P<0.05);湿热模型组大鼠舌黏膜层的角质细胞层、棘细胞层增厚,不完全角化细胞增多;舌固有层组织充血水肿;药物干预组与正常对照组光镜下无明显差别。湿热模型组舌组织AQP-5表达较正常对照组明显增加(P<0.01);药物干预组AQP-5表达较湿热模型组显著减少(P<0.05,P<0.01)。结论:加味藿朴夏苓汤能显著改善温病湿热证模型大鼠腻苔和舌组织病理改变,其机制可能与减轻舌组织炎症,降低AQP-5表达有关。Objective： To explore the effect of modified Huopu Xialing Decoction on AQP-5 expression of tongue tissue in rat with epidemic febrile disease of damp-heat syndrome. Methods： 24 SPF grade SD rats were divided into three groups randomly： normal group, damp-heat syndrome model group, modified Huopu Xialing Decoction group. The model of damp-heat syndrome was established by compound stimulates, observed the changes of tissue under light microscope and the expression of AQP-5 in rat tongue tissue which was analyzed by immunohistochemistry. Results： The damp-heat syndrome model rats of epidemic febrile disease had greasy tongue fur, while the formation of greasy tongue fur remarkably decreased in modified Huopu Xialing Decoction group （P 〈 0.05）. The damp-heat syndrome model group had thicker cutin cellular layer and prickle cell layer, more incomplete keratinocyte ceils than the other groups in tongue mucous membrane. Vasocongestion and edema were also observed in muscular layer. Compared with normal group, model group and Huopu Xialing Decoction group had a remarkable increase in AQP-5 expression. In addition, more AQP-5 expression was found in model group than Huopu Xialing Decoction group（P 〈 0.05）. Conclusion： Modified Huopu Xialing Decoction could ameliorate model rat＇s greasy tongue fur and pathology changes in tongue tissue, which is probably related to tongue tissue inflammation and the AQP-5 expression.

关 键 词：温病湿热证 舌组织 水通道蛋白-5 加味藿朴夏苓汤 

分 类 号：R285.5[医药卫生—中药学]