TGF-β_1氟中毒大鼠肾脏的表达及意义  

Expression of TGF-β1 in the renal tissue of rats

作　　者：万晓军[1] 任鹏[1] 孙发[1] 包成江[1] 陈国志[1] 李洪辉[1] 张耀文[1] 

机构地区：[1]贵阳医学院第二附属医院泌尿外科,贵州凯里55600

出　　处：《吉林医学》2012年第25期5386-5388,共3页Jilin Medical Journal

摘　　要：目的:用腹腔注射氟化钠水溶液的方法建立大鼠急性氟中毒模型,检测氟中毒大鼠肾脏TGF-β1的表达,探讨TGF-β1在氟中毒肾脏损害表达的意义。方法:选择健康成年SD(Sprague-Dawley)大鼠36只,雌雄各半,随机按照注射剂量分组为:高氟组12只(注射氟剂量为20 mg/kg),低氟组12只(注射氟剂量为10 mg/kg),对照组12只(注射0.9%NaCl 5 ml/kg)。采用腹腔注射方式,每2日1次,无氟饮食,分别于饲养后30天剖杀,测定各组大鼠体重差、肾重/体重比,氟中毒大鼠尿常规变化,氟中毒大鼠肾功能变化。离子电极法测量尿氟浓度,测量骨氟浓度,应用免疫组化方法检测肾脏TGF-β1分布和含量,应用HE染色方法显微镜下观察大鼠肾组织结构病理学改变。结果:①大鼠中毒表现:牙齿呈黄白相间的氟中毒体症,且随着染氟剂量的增加,牙齿变化明显,随染氟剂量的增高,尿氟和骨氟浓度增高,各组数据两两比较,P<0.05。②一般指标检测:测定各组大鼠体重差、肾重/体重比,氟中毒大鼠尿常规变化,氟中毒大鼠肾功能变化,各组间比较,P>0.05。③氟中毒大鼠肾组织形态学变化。HE染色:与对照组比较,染氟大鼠主要表现为肾小管上皮细胞水肿、渗出、坏死,细胞间界限不清,且随着染氟浓度增高,损伤改变加重,肾小球和肾间质改变轻微。④TGF-β1在肾小管上皮细胞的胞质中表达,随着染毒浓度增高,表达增强,与对照组相比,差异具有统计学意义(P<0.01)。结论:①表明通过腹腔注射氟化钠氟大鼠制造肾损伤动物模型办法可靠;②氟中毒可引起肾结构损伤,且主要表现为肾小管上皮细胞损伤;③氟中毒TGF-β1主要在大鼠肾脏肾小管表达,可能是肾小管损伤与修复过程中的一对重要互逆平衡因素;④当出现明显氟斑牙等氟中毒表现时,染氟大鼠肾脏功能尚未出现损害,而此时肾脏显微结构已经发生显著改变。在肾功能出现损害前,TGF-β1的表�Objective To detect the TGF -β1expression of fluorosis rats＇ kidney and discuss the meaning of kidney poisoning damage mechanism in fluorosis rats＇ kidney with the model of acute fluoride poisoning rats which were made with the method of intraperitoneal in- jection of sodium fluoride in aqueous solution. Method 36 healthy SD rats, male and female in equal, were randomly divided into high - dose group, 12 rats （injection of fluoride dose to 20 mg/kg）, low dose group, 12 rats （injected dose of fluoride to 10mg/kg）, control group, 12 rats （normal saline 5ml/kg）. Intraperitoneal injection, once every two days,fluorine -free diet. The rats were sacrificed by the same sur- geon after 30 days. Determined the body weight of rats, kidney weight / body weight ratio in each group, change of routine urinalysis and re- hal function of fluorine poisoning rats. Determined the urine fluorine concentration with ion selective electrode, bone fluorine concentration, distribution and content of renal TGF - 131 with immunohisteehemistry. Observed the morphology of rats＇ nephridial tissue under light mi- croscope with HE staining. Results 1. The performance of rats with fluorosis ：Teeth showed yellow and white and it was the symptom offlu- orosis,the teeth changed significantly with the increasing dose of fluoride, fluoride in urine and fluoride in bone concentrations increased with the increasing dose of fluoride. Comparison of each set of data, the difference was significant （ P 〈 0. 05 ）. 2. Detection of general index： measured body weight of rats,kidney weight / body weight ratio in each group ,changes of urine in fluorosis rats,changes of renal function in fluorosis rats, no significant difference among the groups, （ P 〉 0. 05 ）, no statistically significant. 3. Morphological changes of renal tissue in fluorosis rats. Hematoxylin and eosin stain：Compared with the control group,fluorosis rats mainly performaneed that the renal tubular epithe- lial ceils edematous,exudative,necresis,ill -defined

关 键 词：氟中毒 肾 TGF-Β1 病理 

分 类 号：R599[医药卫生—内科学]