病毒灭活血浆对人γδT细胞功能影响的实验研究  

Experimental study of virus inactivated plasma for human γδT cell function influence

作　　者：姚仁南[1] 陈玲[2] 陈娜云[1] 刘军权[2] 周忠海[2] 陈复兴[2] 孙阳[1] 

机构地区：[1]解放军第97医院血站,江苏徐州221004 [2]解放军第97医院中心实验科,江苏徐州221004

出　　处：《生物医学工程与临床》2012年第5期472-476,共5页Biomedical Engineering and Clinical Medicine

基　　金：南京军区医学科技创新重点课题资助(08Z009)

摘　　要：目的探讨病毒灭活血浆对人γδT细胞生长和功能的影响。方法取人外周血γδT细胞,用异戊烯焦磷酸法体外扩增。用10%病毒灭活血浆和10%新鲜冰冻血浆分别培养γδT细胞,分别检测培养前、培养5d和10d后的扩增倍数;用流式细胞术分别检测培养10d后的γδT细胞表面标记,即颗粒酶B、穿孔素和CD107a的表达等。结果 10%新鲜冰冻血浆和10%病毒灭活血浆对人γδT细胞培养10d时,由扩增前的3.12%增加到80.46%和81.18%,5d和10 d的细胞增殖倍数分别为11.65±2.11、38.21±1.57和11.77±2.13、37.11±1.81,CD107a、穿孔素、颗粒酶B含量表达分别为90.54%±1.99%、23.47%±3.18%、35.47%±2.42%和90.22%±2.21%、22.58%±3.41%、34.63%±2.22%,两组比较,差异无统计学意义(P>0.05)。结论病毒灭活血浆在一定浓度下对人γδT细胞生长、增殖,颗粒酶B、穿孔素和CD107a的表达与新鲜冰冻血浆无明显差异。Objective To study the influence of virus inactivated plasma for human γδT cell growth and function. Methods The isopentenyl pyrophosphate assay were used to amplify human peripheral blood γδT cells in vitro. The γδT cells were cultured with 10 % virus inactivated plasma and 10 % fresh frozen plasma, the amplification factors were detected at culture before, after 5 days and 10 days; The flow eytometry were detected cell surface markers, Granzyme B, perforin and CD107a of γδT cultured after 10 days, Results The 10 % fresh frozen plasma and virus inactivated plasma on human γδT cells cultured by amplification at 10 days, which were increased from 3.12 % to 80.46 % and 81.18 %, 5 days and 10 days of cell proliferation multiples were 11.65 ± 2.11, 38.21± 1.57 and 11.77 ± 2.13, 37.11 ± 1.81, respectively. The expression of CD107a, perforin and Granzyme B were 90.54 % ±1.99 % ,23.47 % ± 3.18 %, 35.47 % 4± 2.42 % and 90.22 % 4± 2.21%, 22.58 % ± 3.41%, 34.63 % ± 2.22 %, respectively. There was no statistically significant difference between 2 groups（P 〉 0.05）. Conclusion It is demonstrated that the virus inactivated plasma in certain concentration of human γδT cell growth and proliferation, Granzyme B, perforin and CD107a expression and fresh frozen plasma have no obvious difference.

关 键 词：ΓΔT细胞 病毒灭活血浆 新鲜冰冻血浆 

分 类 号：R457[医药卫生—治疗学]