聚丙烯酰胺凝胶电泳检测亚麻SSR标记  

Polyacrylamide Gel Electrophorus Used in Flax SSR Marker

作　　者：吴建忠[1] 黄文功[1] 赵茜[1] 康庆华[1] 吴广文[1] 关凤芝[1] 赵东升[1] 

机构地区：[1]黑龙江省农业科学院经济作物研究所,黑龙江哈尔滨150086

出　　处：《黑龙江农业科学》2012年第9期18-20,共3页Heilongjiang Agricultural Sciences

摘　　要：以亚麻为试验材料,提取亚麻基因组DNA,选择多态性SSR标记,并将标记产物进行聚丙烯酰胺凝胶电泳,最后硝酸银染色,建立了一套有效、方便的应用于亚麻SSR标记的聚丙烯酰胺凝胶电泳银染检测方法。Taking flax as the test material, extracting flax genome DNA, selecting the polymorphic SSR mark- ers, PCR products were electrophoresised in the polyacrylamide gel,and finally they were stained with silver ni- trate. An effective,simple and quick PAGE silver staining method was built by flax SSR markers.

关 键 词：PAGE 亚麻 SSR 

分 类 号：S563.2[农业科学—作物学]