遗传转化中诱导蓖麻子叶节分化适宜的PGR条件  

Appropriate PGR Conditions Inducing Differentiation of Castor Cotyledonary Node under Genetic Transformation Condition

作　　者：王文跃[1,2] 陈永胜[1,2] 李国瑞[1,2] 黄凤兰[1,2] 尚雨丝[1] 王超[1] 张智勇[1] 

机构地区：[1]内蒙古民族大学,内蒙古通辽028000 [2]内蒙古自治区高校蓖麻产业工程研究中心,内蒙古通辽028000

出　　处：《山西农业科学》2012年第9期925-929,共5页Journal of Shanxi Agricultural Sciences

基　　金：国家自然科学基金项目(30760123;31160290);教育部新世纪优秀人才支持计划(NCET-08-0870);内蒙古自然科学基金项目(2009BS0304;2010BS0511);国家民委项目(10NM02)

摘　　要：以通蓖5号蓖麻子叶节为试材,应用组织培养技术以及不同质量浓度的ZT和NAA,IAA,IBA处理农杆菌浸染后的子叶节,以期寻找其共培养后诱导芽及根适宜的PGR质量浓度配比,建立蓖麻遗传转化中的快繁体系。结果表明,当ZT质量浓度为2.0 mg/L时,最适合蓖麻子叶节芽诱导;IBA为最适根诱导剂,最适质量浓度为0.10 mg/L。Taking castor cotyledonary node of Tongbi 5 as test material and using tissue culture techniques and different concentrations of ZT and the cotyledons of NAA, IAA, IBA was conducted by agrobacterium in this study to find common culture induced bud and root appropriate PGR concentration ratio of castor-oil plant genetic transformation micropropagation system. The results showed that： ZT concentration of 2.00 mg/L was the optimal for castor cotyledonary node induction; IBA was the optimum root inducing agents, and the optimal induction of root concentration was 0.10 mg/L.

关 键 词：蓖麻子叶节 PGR浓度 诱导芽 诱导根 

分 类 号：S565.6[农业科学—作物学]