傅立叶变换红外光谱技术对2种葡萄球菌的快速分类鉴定  

Rapid Differentiation and Identification of Staphylococcus aureus and Staphylococcus epidermidis by FT-IR spectroscopy

作　　者：李兆杰 王静 隋涛 宋晓华 刘玉敏 崔凤杰 杨丽君 

机构地区：[1]威海出入境检验检疫局,山东威海264205 [2]烟台出入境检验检疫局,山东烟台264000

出　　处：《安徽农业科学》2012年第27期13226-13227,共2页Journal of Anhui Agricultural Sciences

基　　金：国家质量监督检验检疫总局科研项目(2010IK151)

摘　　要：[目的]建立金黄色葡萄球菌和表皮葡萄球菌2种菌的傅立叶变换红外光谱数据库及FT-IT分类鉴定方法。[方法]应用FT-IR技术对2种葡萄球菌进行指纹图谱数据采集,应用化学计量学分析方法对光谱进行分析。[结果]建立2种葡萄球菌的标准FT-IR光谱数据库,用于FT-IR技术对2种可疑目标葡萄球菌进行鉴定;建立一种基于主成分分析的聚类分析模型,可成功地区分2种葡萄球菌。[结论]傅立叶变换红外光谱分析方法简便、快速、易操作,结果重现性好,是一种区分金黄色葡萄球菌和表皮葡萄球菌的有效方法。[Objective] The research aimed to establish a standard Fourier transform infrared（FT-IR） spectral library and a FT-IR method of differentiation and identification of Staphylococcus aureus and Staphylococcus epidermidis.[Method] FT-IR fingerprint absorption spectra of two species of Staphylococcus were collected and analyzed by chemometric methods.[Result] A standard FT-IR spectral library was created and could be used to compare with those of the target Staphylococcus and identify them.A cluster model of principal component analysis was established and could differentiate and identify well the two species of Staphylococcus.[Conclusion] As a rapid,easy-to-use and accurate technique,FT-IR spectroscopy is an effective tool to differentiate and identify two species of Staphylococcus.

关 键 词：傅立叶变换红外光谱 金黄色葡萄球菌 表皮葡萄球菌 分类 鉴定 

分 类 号：S123[农业科学—农业基础科学]