聚乙二醇干扰素治疗非活动性HBsAg携带者获得HBsAg清除者的CD4^+CD25^+调节T细胞的研究  

CD4^+CD25^+ Regulatory T Cells From Peripheral Blood in Inactive HBsAg Carriers Following Peginterferon Therapy

作　　者：于海滨[1] 马丽娜[1] 柳雅立[1] 画伟[1] 何志敏[1] 鲁俊锋[1] 陈新月[1] 

机构地区：[1]首都医科大学附属北京佑安医院,100069

出　　处：《医学研究杂志》2012年第8期29-31,共3页Journal of Medical Research

基　　金：国家"十一五"科技重大专项课题基金资助项目(2008ZX10002-004)

摘　　要：目的探讨聚乙二醇干扰素治疗非活动性HBsAg携带者过程中CD4+CD25+Treg的意义。方法 15例非活动性HBsAg携带者接受聚乙二醇干扰素-α2a,治疗至48周全部获得了HBsAg清除。每12周观察肝功能、乙肝表面抗原效价、CD4+CD25+Treg变化。结果 HBsAg清除前节点的ALT(59.41±35.20U/L)较基线(25.72±12.85U/L)、48周(27.86±10.27U/L)升高更明显(P<0.05);CD4+CD25+Treg变化与ALT同步,HBsAg清除前节点的ALT(9.07±3.37)较基线(2.72±1.33)、48周(3.13±1.18)升高更明显(P<0.05);谷丙转氨酶(ALT)与CD4+CD25+Treg比率呈正相关(R=0.769,P=0.008)但是Treg比率与HBsAg效价(log)无相关性(R=-0.14,P=0.879)。结论聚乙二醇干扰素-α2a治疗过程中,ALT在HBsAg清除前节点明显升高,可能系机体免疫被激活表现,而CD4+CD25+Treg比率同步升高可使机体维持保护性免疫,避免激发过度的免疫反应所致的肝脏病理损伤。Objective To investgate CD4 ^＋ CD25 ＋ regulatory T cells from peripheral blood in inactive HBsAg carriers following PEG - interferon - α 2a treatment. Methods Fifteen patients received PEG - interferon - α 2a treatment for 48 weeks and achived HBsAg clearance . All subjects were assessed for HBsAg titler,presence of anti - HBe, serum alanine aminotransaminase, the propotion of CD4 ^＋ CD25 ^＋ regulatory T ceils per 12weeks. Results The ALT level and propotion of CD4 ^＋ CD25 ＋ regulatory T ceils at pre - seroclearance point were higher than those at baseline and weeks 48. ALT was positively correlated with the propotion of CD4 ^＋ CD25 ＋ regulatory T cells. ALT was not related with the propotion of CD4 ^＋ CD25 ^＋ regulatory T cells. Conclusion The increase of ALT at pre - seroclearance point is a suggestion of the intensive immunity in patients with chronic hepatitis B, which could be related to the hepatocyte damage. While the CD4 ＋ CD25 ＋ Treg ratio rise accordingly can avoid excessive immune responses those may induce liver pathological injury.

关 键 词：非活动性HBSAG携带者 聚乙二醇干扰素-α2a 调节性T细胞 

分 类 号：R512.62[医药卫生—内科学]