脑出血患者抵抗素基因+299G>A位点多态性分析  

+299G>A Polymorphism of Resistin Gene in the Patients with Intracerebral Hemorrhage

作　　者：董晓巧[1] 杜权[1] 俞文华[1] 张祖勇[1] 陈隽 翁建丰 朱强[1] 车志豪[1] 

机构地区：[1]杭州市第一人民医院神经外科,310006 [2]慈溪市第二人民医院神经外科,315315

出　　处：《医学研究杂志》2012年第8期82-85,共4页Journal of Medical Research

基　　金：杭州市医药卫生科技计划项目(2010A009)

摘　　要：目的揭示浙江地区汉族脑出血患者抵抗素基因+299G>A位点单核苷酸多态性与血浆抵抗素及C-反应蛋白水平的相关性。方法选取344例高血压性基核出血患者和344例年龄及性别均配对的健康体检者,入院时或体检时抽取外周血提取DNA,采用聚合酶链反应-限制性片段多态性分析基因型,同时采用ELISA法检测脑出血患者血浆抵抗素及C-反应蛋白浓度,统计分析其相关性。结果酶切后可见抵抗素基因+299G>A位点有GG、GA和AA 3种基因型。健康体检者等位基因G和A的频率分别为64.8%和35.2%,基因型GG、GA和AA的频率依次为41.9%、45.9%和12.2%。脑出血患者等位基因G和A的频率分别为69.3%和30.7%,基因型GG、GA和AA的频率依次为为49.1%、40.4%和10.5%。经Hardy-Weinberg遗传平衡定律检验,脑出血患者及健康体检者各基因型频率均符合遗传平衡。经卡方检验,脑出血患者及健康体检者各基因型差异无统计学意义(P=0.159)。GG、GA和AA基因型脑出血患者血浆抵抗素浓度依次为(23.9±7.2)ng/ml、(26.4±7.2)ng/ml和(26.9±9.7)ng/ml,血浆C-反应蛋白浓度依次为(7.5±2.2)mg/L、(8.3±2.7)mg/L和(8.4±3.0)mg/L。采用方差检验,GG基因型患者血浆抵抗素和C-反应蛋白浓度均显著低于GA和AA基因型患者(P均<0.05),而GA和AA基因型患者血浆抵抗素和C-反应蛋白浓度差异无统计学意义(P均>0.05)。采用多元线性回归分析,脑出血患者入院时血浆抵抗素浓度与C-反应蛋白浓度显著正相关(t=7.032,P<0.001)。结论浙江地区汉族脑出血患者抵抗素基因+299G>A位点单核苷酸多态性与血浆抵抗素和C-反应蛋白浓度显著相关,可能参与脑出血炎症反应。Objective This study aimed to examine the relations of the ± 299G 〉 A single nucleotide polymorphism of resistin gene to the plasma resistin and C - reactive protein concentrations in intracerebral hemorrhage. Methods Totally 344 Chinese Han patients with intracerebral hemorrhage and 344 age - and sex - matched healthy subjects were included in this study. Plasma resistin and C - reac- tive concentrations were measured in the patients, as well as ± 299G 〉 A polymorphisms of resistin gene in the healthy subjects and pa- tients were genotyped using PCR - restriction fragment length pnlymorphism. Results The genotypes of the ± 299G 〉 A polymorphism of resistin gene included GG, GA and AA. The G and A allele frequencies of the healthy subjects were 64.8% and 35.2% , respectively. The genotype GG, GA and AA frequencies of the healthy subjects were 41.9% , 45.9% and 12.2% , respectively. The G and A allele frequencies of the patients were 69. 3% and 30.7% , respectively. The genotype GG, GA and AA frequencies of the patients were 49. 1% , 40.4% and 10.5% , respectively. The genotype frequencies of the ± 299G 〉 A polymorphism of resistin gene in the healthy sub- jects and patients were in the Hardy -Weinberg equilibrium. The genotype frequencies between healthy subjects and patients showed no significant differences （ P = 0. 159）. Plasma resistin levels of the patients with the genotype GG, GA and AA were （23.9 ± 7.2 ） ng/ml, （ 26.4 ± 7.2） ng/ml and （26.9 ± 9.7） ng/ml respectively, as well as their C - reactive protein levels were （ 7.5 ± 2.2 ） mg/L, （ 8.3 ± 2.7） mg/L and （8.4 -± 3.0）mg/L respectively. By analysis of variance, the common homozygote （GG） had the lowest resistin and C - reactive protein plasma concentrations （P all 〈 0.05） ; the plasma resistin and C - reactive protein concentrations between heterozygote （ GA ） and rare allele homozygote （AA） did not differ significantly （ P all 〉 0.05 ）. Plasma resistin level was significantly associat

关 键 词：抵抗素 基因多态性 脑出血 C-反应蛋白 炎症反应 

分 类 号：R978.19[医药卫生—药品]