建立大型犬骨质疏松动物模型的实验研究  

Study on Establishment of Large Canine Osteoporosis Model

作　　者：王方[1] 周建伟[1] 张瑞娟[1] 曾辉[1] 邹德威[1] 吴继功[1] 马华松[1] 

机构地区：[1]北京大学解放军306医院教学医院骨科,100191

出　　处：《医学研究杂志》2012年第8期134-137,共4页Journal of Medical Research

基　　金：总后勤部"十一五"专项基金资助项目(08Z018)

摘　　要：目的采用激素注射联合低钙饮食的方法快速建立大型犬骨质疏松动物模型。方法健康雌性杂种犬16只,体重27.0±1.8kg,5～7岁龄,随机分两组(每组8只):实验组喂低钙饲料,静脉注射地塞米松磷酸钠注射液2.5mg/kg,每周2次;对照组喂食正常犬饲料,静脉注射生理盐水2.5ml/kg,每周2次。实验前、实验4周、8周进行骨密度测定、生化检查,实验12周进行骨密度测定、生化检查、骨生物力学及骨组织形态学检查。结果与对照组相比,实验组应用激素合并低钙饮食8周骨密度显著降低(P<0.05),12周进一步降低(P<0.01);实验中两组血钙无显著差别(P>0.05),实验组4周血磷较对照组降低(P<0.05),8周及12周血磷进一步降低(P<0.01),实验4、8、12周实验组碱性磷酸酶均显著升高(P<0.01);实验组第12周骨小梁面积百分数(%Tb.Ar)、骨小梁厚度(Tb.Th)、骨小梁数量(Tb.N)较对照组显著降低(P<0.01),骨小梁分离度(Tb.Sp)较对照组显著增高(P<0.01);实验第12周实验组腰椎压缩极限载荷、极限强度和压缩模量均明显低于对照组(P<0.01),股骨三点弯曲极限载荷实验组低于对照组(P<0.05)。结论激素注射联合低钙饮食的方法短期内成功建立了大型犬骨质疏松动物模型,该模型具有人类骨质疏松症的临床特性,为进行外科学实验研究提供了依据。Objective To establish large canine osteoporosis model quickly using the method of both hormone injections and low cal- cium diet. Methods Totally 16 healthy female hybrid canine,which weighted 27.0 ± 1.8kg and were 5 -7 years old,were divided into two groups （each group 8）. Experimental group was intravenous injected dexamethasone sodium phosphate injection 2.5mg/kg twice per week, and fed low calcium feed. Control group was injected physiological salinetwice per week, and fed normal feed. The bone mineral density and biochemical indicator were measured both before the experiment and on the 4th, 8th, and 12th week of the experiment. The biomechanics of bone and bone tissue morphology were examined on the 12th week of the experiment. Results On the 8th week of experi- ment, bone mineral density significantly reduced （P 〈 0.05 ） , and further reduced （ P 〈 0.01 ） on the 12th week. During the experimental process ,the calcium of the two group had no significant difference（ P 〉 0.05 ）. On the 4th week the phosphorus of the experiment group re- duced（P 〈0.05） ,and further reduced（P 〈0.01 ）on the 8th and 12th week. On the 4th, 8th and 12th week the alkaline phosphatase of the experiment group significantly increased（ P 〈 0.01 ）. On the 12th weeks the Tb. Ar,Tb. Tb and Tb, N of the experiment group were sig- nificantly reduced and the Tb. Sp was significantly incrreased（P 〈 0.01for both）comparing with the control group. On the 12th week the lumbar compressive ultimate load, ultimate strengthand compression modulus of the experiment group were significantly lower than the con- trol group （ P 〈 0.01 ） , the 3 point bending ultimate load of the experiment group was lower than the control group（ P 〈 0.05 ）. Conclusion The method of both hormone injections and low calcium diet successfully established large osteoporosis animal model in the short term, and this model has human osteoporosis clinical characteristics, providing the basis for surgical experimental resea

关 键 词：糖皮质激素 骨质疏松 低钙饮食 动物模型 

分 类 号：R817.5[医药卫生—影像医学与核医学]