急性暴饮性饮酒对小鼠脑损伤生物标志物的影响  

Biomarkers of Alcohol Binge-drinking Induced Brain Damage in Mice

作　　者：赵美清[1] 冯利东[2] 

机构地区：[1]内蒙古医学院第三附属医院,包头014010 [2]包钢第三职工医院

出　　处：《医学研究杂志》2012年第8期169-171,共3页Journal of Medical Research

摘　　要：目的研究急性暴饮性饮酒对小鼠脑组织中氧自由基相关生物学标志物的影响。方法首先建立急性暴饮性饮酒的小鼠动物模型,检测其血液中乙醇的浓度,进行大脑病理解剖学检查,并与正常对照组比较;然后测定该动物模型以及正常动物脑组织中超氧化物歧化酶(SOD)的活性以及丙二醛(MDA)的含量水平。结果在单次乙醇胃灌注小鼠大脑并没有观察到明显的显微镜下病理学变化,然而单次乙醇胃灌注可以使小鼠脑组织中SOD的活力显著降低(P<0.01);而使MDA含量水平显著升高(P<0.01)。结论一次性大量饮酒可以导致小鼠大脑氧自由基相关生物学标志物水平的变化,表现为降低降解自由基酶的活力和升高氧自由基的水平,从而可能造成脑损伤。Abstract Objective To study oxyradical - related biomarkers for binge - drinking inducing alcohol - induced brain damagein mice. Methods A binge drinking model was created with a single dose of alcohol that was administered by gavage to mice. Blood al- cohol levels were monitored and the brain biopsy was performed for the pathological examination, and compared with the normal control group. The activity of SOD and the level of MDA in the mice brain were measured between the two groups of animals. Results There was no observable microscopic change in the brain after a single dose of alcohol exposure. However, the activity of SOD in the mice brain de- creased and the level of MDA increased dramatically （ P 〈 0.01 ）. Conclusion A single dose of alcohol binge - drinking can cause oxyradical - related biomarkers change in brain which expressed as increased oxygen radicals and decreased degradation enzymes of perox- ides, resulting in brain damage.

关 键 词：急性酒精性脑损伤 小鼠 动物模型 生物标志物 

分 类 号：R963[医药卫生—微生物与生化药学]