应用改良四动脉结扎法制作猫全脑缺血再灌注损伤模型  

Improved four-vessel occlusion method in establishing a cat model of global brain ischemia and reperfusion injury

作　　者：林绿标[1] 林旭妍[2] 许益民[1] 肖哲[1] 

机构地区：[1]汕头大学医学院第一附属医院神经外科,广东省汕头市515041 [2]汕头大学医学院第一附属医院眼科,广东省汕头市515041

出　　处：《中国组织工程研究》2012年第33期6169-6172,共4页Chinese Journal of Tissue Engineering Research

基　　金：广东省医学科研基金资助项目(A2010395)~~

摘　　要：背景:四血管结扎法制作全脑缺血模型目前主要用于小鼠、大鼠等小型动物,大型动物模型方面鲜有报道,缺少有关的解剖资料。目的:探讨四动脉结扎法制作猫全脑缺血再灌注损伤模型的方法改良。方法:参照Pulsinelli四动脉结扎法,提出电凝位于翼突与第二颈椎横突之间的椎动脉,永久性阻断双侧椎动脉。再临时阻断双侧颈总动脉,以动态脑电图监测出现静息脑电波为判定猫全脑缺血的标准。15min后恢复颈动脉供血,进行再灌注损伤。假手术组猫不进行电凝或夹闭即缝合切口。结果与结论:模型制作成功25只,模型制作不成功1只,死亡4只,成功率83%。苏木精-伊红染色示缺血脑组织呈典型的迟发性神经元坏死。提示改进后的四血管法制作猫脑缺血再灌注损伤模型,效果确切,模型制作成功率高。BACKGROUND： At present, Pulsinelli four-vessel occlusion method is mainly applied to prepare global brain ischemia and reperfusion injury models in mice, rats and other small animals. It is little used in the preparation of big animal model., and the relative anatomical documentation is of deficiency. OBJECTIVE： To explore the improved four-vessel occlusion method for preparation of global brain ischemia and repeffusion injury models of cats. METHODS： Referring to Pulsinelli four-vessel occlusion method, reforming of operation was proposed that bilateral vertebral arteries between the pterygoid process and transverse process of axes were blocked forever by electric coagulation. After that, bilateral carotid arteries were blocked temporarily. When resting brain wave appeared on active electroencephalogram, the brains of cats were considered as global ischemia. After 15 minutes of ischemia, the brains o cats were impaired by repeffusion. Arteries of cats in sham operated group were exposed but not electrically coagulated or blocked. RESULTS AND CONCLUSION： Twenty-five models were successful, one was unsuccessful, and four cats were dead The achievement ratio was 83%. The appearance of ischemic brain tissues was typical tardive neuron necrosis. The improved four-vessel occlusion method is effective to establish global brain ischemia and reperfusion injury models in cats with high achievement ratio.

关 键 词：脑缺血 再灌注损伤 模型 四血管阻塞 猫 

分 类 号：R318[医药卫生—生物医学工程]