羟基丁酸-羟基戊酸纳米纤维材料修复胫骨缺损  

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) nanofibers for repairing rabbit bone defects

作　　者：叶荣 张晓峰 严怀宁 潘永飞 黄宁平[2] 吕兰欣[2] 蒋赞利[3] 

机构地区：[1]武警江苏省总队南京医院骨科,江苏省南京市210028 [2]东南大学生物科学与医学工程学院,生物电子学国家重点实验室,江苏省南京市210096 [3]东南大学附属中大医院骨科,江苏省南京市210009

出　　处：《中国组织工程研究》2012年第34期6284-6288,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:羟基丁酸-羟基戊酸纳米纤维材料促进骨组织再生的体内实验研究较少,且研究结果有争论。目的:观察羟基丁酸-羟基戊酸纳米纤维材料修复骨缺损的能力。方法:随机选取45只新西兰兔,制备单侧胫骨中上段骨缺损模型,随机均分为3组,实验组植入羟基丁酸-羟基戊酸纳米纤维材料,对照组植入β-磷酸三钙人工骨,空白组不植入任何材料。在术后相应时间段分别进行X射线摄片,组织学检查,扫描电镜观察其成骨能力、生物降解性、生物相容性等指标。结果与结论:术后4～8周,实验组骨缺损植入材料处片状密度增强,截骨两端骨质向缺损区生长,骨皮质不连续,仍见骨性缺损,术后8～12周,新生皮质骨与宿主皮质骨自然连接,骨缺损完全修复。术后4,8周,实验组与对照组在新生骨的形成及修复骨缺损方面相差不明显(P>0.05),12周时,实验组具备更好的成骨能力,与对照组比较差异有显著性意义(P<0.05)。结果可见羟基丁酸-羟基戊酸纳米纤维材料具有良好的生物相容性及骨传导性,是一种良好的骨组织工程支架材料。BACKGROUND： The experimental studies about poly （3-hydroxybutyrate-co-3-hydroxyvalerate） （PHBV） nanofibers for bone tissue regeneration are few and the research results have disputes. OBJECTIVE： To investigate the capability of PHBV nanofibers for repairing bone defects METHODS： Totally 45 New Zealand white rabbits were selected. A rabbit model of bone defects in middle upper section of unilateral tibia was established. The rabbits were evenly randomized into three groups. The experimental group was implanted with PHBV nanofibers, and the control group was implanted with β-tricalcium phosphate, while nothing was implanted into the blank group. Bone formation capability, biodegradation, biocompatibility were evaluated by gross observation, radiology, histology examination and scanning electron microscope observation at corresponding time period postoperatively. RESULTS AND CONCLUSION： At weeks 4 8 after implantation, the density of the bone defect regions was increased, osteotylus generated from osteotomy sides grew into defect region; new cortical bone was not continuous and bone defects still remained. At weeks 8 12 after implantation, new cortical bone was connected with the host cortical bone naturally, and the bone defects were perfectly healed. There was no significant difference between the experimental group and control group in new bone formation and bone defects repair at weeks 4 and 8 postoperatively （P 〉 0.05）. At week 12 postoperatively, the capability of bone formation in the experimental group was significantly better than that in the control group, and there was a significant difference between the two groups （P 〈 0.05）. These findings suggest that PHBV nanofibers exhibit good biocompatibility and osteoconduction, and can be used as an ideal scaffold for bone tissue engineering.

关 键 词：羟基丁酸-羟基戊酸 纳米纤维材料 胫骨缺损 Β-磷酸三钙 骨组织再生 生物材料 

分 类 号：R318[医药卫生—生物医学工程]