无机三氧化物聚合物盖髓材料对成牙本质样细胞的毒性  

Cytotoxicity of mineral trioxide aggregate to odontoblast-like cells

作　　者：王健平[1] 王兆宏[2] 贾若恬[1] 冯瑶[1] 

机构地区：[1]佳木斯大学附属口腔医院,黑龙江省佳木斯市154002 [2]黑龙江省卫生厅,黑龙江省哈尔滨市150000

出　　处：《中国组织工程研究》2012年第34期6331-6334,共4页Chinese Journal of Tissue Engineering Research

摘　　要：背景:无机三氧化物聚合物是一种优良的盖髓材料,它对原代培养成牙本质细胞的细胞毒性研究较少。目的:通过与氢氧化钙制剂比较,检测无机三氧化物聚合物对小鼠原代培养成牙本质样细胞的细胞毒性。方法:取19d龄胎鼠的下颌第一磨牙牙胚,分离牙乳头组织,用组织块培养法培养;然后用滤纸片法挑选出与成牙本质细胞形态接近的细胞继续培养,对挑选出的细胞从形态学观察及牙本质涎磷蛋白在mRNA水平上的表达方面进行鉴定。将培养出的成牙本质样细胞与含有无机三氧化物聚合物或氢氧化钙制剂的DMEM培养基或空白DMEM培养基共同培养2,4,6d后,通过CCK-8技术检测体外培养成牙本质样细胞的毒性。结果与结论:镜下观察培养的细胞呈梭形、长三角形或梨形,有单侧较长突起,并特异性表达牙本质涎磷蛋白。细胞毒性方面,无机三氧化物聚合物组随培养时间延长,细胞数目逐渐增多(P<0.05),细胞增殖良好;氢氧化钙制剂组细胞增殖情况显著低于空白对照组(P<0.05)。提示与氢氧化钙制剂相比,无机三氧化物聚合物对成牙本质样细胞有较小的细胞毒性,具有良好的生物相容性。BACKGROUND：Mineral trioxide aggregate（MTA） is a fine pulp capping material.But there are few researches about the cytotoxicity of MTA to odontoblasts in primary culture.OBJECTIVE：To investigate the cytotoxicity of MTA to mouse odontoblast-like cells in primary culture compared with Dycal.METHODS：The mandibular first molar tooth germs from 19 days fetal rats were taken out.The dental papillae was isolated and cultured by using tissue block culture.The dental papillae cells which had similar morphology with odontoblasts were selected by filter paper and cultured continually.The cells were identified by morphology observation and the mRNA expression of dentin sialophosphoprotein.The cytotoxic of odontoblast-like cells cultured in vitro was detected by using Cell Counting Kit-8 assay,after the cultured odontoblast-like cells were co-cultured with the blank DMEM culture medium or the culture medium containing MTA or Dycal for 2,4 and 6 days.RESULTS AND CONCLUSION：Under microscope observation,the cultured cells showed fusiform,long triangle and pyriform,and unilateral longer protuberance.Besides,the cells had specific dentin sialophosphoprotein expression.In terms of cytotoxicity,the number of cells in MTA group was increased gradually with the culture time prolonged（P 〈 0.05）,and the cell proliferation was good.The cell proliferation in Dycal group was significantly less than that in the blank control group（P 〈 0.05）.These findings suggest that compared with the Dycal,the cytotoxicity of MTA to odontoblast-like cells is smaller.MTA has good biocompatibility.

关 键 词：无机三氧化物聚合物 成牙本质样细胞 细胞培养 细胞鉴定 细胞毒性 

分 类 号：R318[医药卫生—生物医学工程]