兔许旺细胞与猪小肠黏膜下层的体外复合培养  

Rabbit Schwann cells co-cultured with swine small intestinal submucosa in vitro

作　　者：魏人前[1] 曹兴海[1] 涂大华[1] 

机构地区：[1]佛山市第二人民医院骨科,广东省佛山市528322

出　　处：《中国组织工程研究》2012年第34期6349-6353,共5页Chinese Journal of Tissue Engineering Research

基　　金：广东省医学科研基金立项课题项目(A2010601);佛山市科技厅项目(201008026)~~

摘　　要：背景:国内外研究以大鼠许旺细胞与小肠黏膜下层复合修复神经缺损,取得了较好结果。目的:观察兔许旺细胞与猪小肠黏膜下层体外共培养的生物相容性。方法:采用分步酶消化法分离培养新西兰大白兔许旺细胞,取第3代许旺细胞接种在猪小肠黏膜下层上。结果与结论:①苏木精-伊红染色:复合培养24h后细胞在材料上良好黏附。1周时部分细胞在猪小肠黏膜下层基质层表面呈单层生长,细胞扁平,细胞核呈长梭形,细胞间连接紧密。2周后,细胞呈多层生长。②扫描电镜:复合培养2d,细胞黏附于材料表面并伸展,细胞体呈纺锤形,从胞体伸出两根细长突起,相邻细胞的突起首尾相接连成细胞链或融合或交联或在纤维的侧方平行生长;1周后细胞在材料上大量增殖,呈串珠链黏附于支架上,类似于神经中的Bunger带。表明小肠黏膜下层与许旺细胞有良好的相容性。BACKGROUND：Studies have shown that the repair of nerve defects by rabbit Schwann cells combined with swine small intestinal submucosa composite have obtained good results.OBJECTIVE：To investigate the biocompatibility of in vitro co-culture of rabbit Schwann cells and swine small intestinal submucosa.METHODS：Schwann cells from New Zealand white rabbits were separated and cultured by step-by-step enzyme digestive method.Passage 3 Schwann cells were selected and seeded on swine small intestinal submucosa.RESULTS AND CONCLUSION：①Hematoxylin-eosin staining：After Schwann cells co-cultured with small intestinal submucosa for 24 hours,cells showed good adhesion.Some cells displayed monolayer growth on the surface of stroma in swine small intestinal submucosa;besides,the cells were flat,long spindle-shaped and connected closely with each other.After 2 weeks,the cells showed multilayer growth.②Scanning electron microscope：After 2 days,Schwann cells adhered on the surface of co-cultured small intestinal submucosa and stretched.The cells displayed spindle-shaped,and stretched out two slender processes,besides,the adjacent cell processes were connected end to end and formed a chain of cells or fusion or cross-link or parallel growth in the fiber side.After 1 week,the cells proliferated greatly and showed a beaded chain adhered on the material,which was similar to the Bunger band of nerve.These findings suggest that small intestinal submucosa and Schwann cells have good compatibility.

关 键 词：许旺细胞 小肠黏膜下层 复合培养 组织工程 神经 生物材料 

分 类 号：R318[医药卫生—生物医学工程]