牙种植体与许旺细胞联合培养时脑源性神经营养因子及神经生长因子mRNA的表达  

Expression of brain-derived neurotrophic factor and nerve growth factor mRNA in the co-culture of schwann cells and dental implants

作　　者：孙兰英[1] 李肇元[2] 葛文章[1] 王其宝[3] 王浩[4] 

机构地区：[1]济南市口腔医院修复科,山东省济南市250001 [2]济南市口腔医院口腔颌面外科,山东省济南市250001 [3]济南市口腔医院内科,山东省济南市250001 [4]复旦大学上海医学院基础实验中心,上海市200032

出　　处：《中国组织工程研究》2012年第34期6372-6376,共5页Chinese Journal of Tissue Engineering Research

基　　金：济南市卫生科技发展计划资助项目(2008-30);济南市科技发展计划资助项目(2008-070280)~~

摘　　要：背景:许旺细胞很可能是促进骨整合种植牙周围神经再生的有效因子。目的:观察许旺细胞与牙种植体复合培养时脑源性神经营养因子与神经生长因子mRNA的表达,分析牙种植体与许旺细胞的相容性。方法:取成年犬发生Wallerian变性的坐骨神经,用酶消化法和差速贴壁法相结合培养许旺细胞,以1×108L-1浓度接种于喷钛砂酸蚀的牙种植体表面和培养皿。分别在细胞培养的第1,3,6,8,11天,以反转录-聚合酶链反应检测许旺细胞中脑源性神经营养因子与神经生长因子mRNA的表达。结果与结论:许旺细胞与牙种植体复合培养时,脑源性神经营养因子与神经生长因子的表达趋势与许旺细胞单独培养时相同,呈单峰型;但两种基因的表达水平高于许旺细胞单独培养组,且达到表达峰值的时间较早。提示喷钛砂酸蚀表面的牙种植体能够促进许旺细胞的基因表达,两者具有良好的相容性。BACKGROUND：Schwann cells are probably the effective factors which can promote peripheral nerve regeneration of osseointegrated dental implants.OBJECTIVE：To investigate the expression of brain-derived neurotrophic factor（BDNF） and nerve growth factor（NGF） mRNA in the co-culture of Schwann cells and dental implants,and to analyze the biocompatibility of dental implants and Schwann cells.METHODS：Wallerian degenerated sciatic nerves were harvested from adult dogs.Schwann cells were cultured by enzymatic digestion and differential adhesion methods.1×108/L cells culture was then seeded onto the dental implants with titanium sandblasted and acid-etched surface as well as petri dish.The expression of BDNF and NGF mRNA in Schwann cells was detected by reverse-transcription PCR at days 1,3,6,8 and 11,respectively.RESULTS AND CONCLUSION：BDNF and NGF mRNA had a similar expression pattern with one peak either in the co-cultured Schwann cells or Schwann cells cultured alone.However,compared with the Schwann cells cultured alone,the expression levels of BDNF and NGF mRNA were higher and earlier peak appeared in the co-cultured Schwann cells.These findings suggest that dental implants with titanium sandblasted and acid-etched surface can promote the gene expression of Schwann cells.There is an excellent biocompatibility between this kind of dental implants and Schwann cells.

关 键 词：牙种植体 许旺细胞 脑源性神经营养因子 神经生长因子 复合培养 

分 类 号：R318[医药卫生—生物医学工程]