多重耐药大肠埃希菌氨基糖苷类修饰酶与Ⅰ类整合酶基因的研究  

Research on an anminoglycosides modifying enzyme genes and intI1 gene in Escherichia coli

作　　者：吴志鹃[1] 游春芳[1] 黄永茂[1] 陈枫[2] 钟利[1] 向成玉[3] 陈庄[2] 

机构地区：[1]四川泸州医学院附属医院感染科,泸州646000 [2]四川泸州医学院附属医院医学实验中心,泸州646000 [3]四川泸州医学院附属医院检验科,泸州646000

出　　处：《中国抗生素杂志》2012年第9期702-705,720,共5页Chinese Journal of Antibiotics

基　　金：四川省重点学科重点建设项目资助(No.SZD0241)

摘　　要：目的探讨本地区临床分离多重耐药大肠埃希菌(Escherichia coli,E.coli)的氨基糖苷类修饰酶和I类整合子基因存在情况。方法纸片扩散法(K-B法)测定71株大肠埃希菌对常用抗菌药物的耐药性,应用PCR技术对E.coli进行氨基糖苷类修饰酶和I类整合酶基因的检测。结果 71株大肠埃希菌对庆大霉素、左氧氟沙星、头孢噻肟、头孢他啶的耐药率分别分为66.20%、63.38%、59.15%、18.31%,其中23株为多重耐药株(32.39%)。氨基糖苷类修饰酶基因aac(3)-II阳性40株(56.34%),aac(6')-I阳性22株(30.99%),ant(3")-I阳性14株(19.72%),未检出aac(6')-II阳性株,氨基糖苷类修饰酶基因总检出率为74.65%;I类整合酶基因(intI1)阳性53株(74.65%)。多重耐药与非多重耐药大肠埃希菌中aac(3)-II、aac(6')-I、总氨基糖苷类修饰酶基因、intI1的阳性率有统计学差异(P<0.05)。结论本地区多重耐药E.coli的氨基糖苷类修饰酶基因、I类整合酶基因携带率高。Objective To investigate the anminoglycosides modifying enzyme genes and intI1 gene in Escherichia coli. Methods The susceptibilities of 71 E. coli strains to 4 antibiotics were detected by disk diffusion test which was recommended by NCCLS 2006. The anminoglycosides modifying enzyme genes and intI1 gene were detected by PCR. Results 47 strains （66.20%）were resistant to gentamicin, 45 strains （63.38%） to levofloxacin, 42 strains （59.15%） to eefotaxime, and 13 strains（18.3 1%）to ceftazidime. There were 23 （32.39%） multidrug resistant strains in 71 E. coli strains. In 71 strains of E. coli, the positive strains of aac（3）-II were 40 strains （56.34%）, aac（6＇）-I were 22 strains （30.99%）, ant（3＂）-I were 14 strains （19.72%）, and aac（6＇）-II positive strains were not detected. The total detection rate of anminoglycosides modifying enzyme genes was 74.65%. The positive strains of intI1 gene was 53 strains（74.65%）. There was statistical difference between the positive rate of multidrug resistant and nonmultidrug resistant strains. Conclusion There are high detection rates of the anminoglycosides modifying enzyme genes and intII gene in the multidrug resistant E. coli strains isolated from the hospital.

关 键 词：大肠埃希菌 多重耐药 氨基糖苷类修饰酶基因 I类整合酶基因 

分 类 号：R378.2[医药卫生—病原生物学]