不同浓度异氟醚对小鼠肺表面活性蛋白A表达的影响  

Effect of Isoflurane with different concentrations on the expression of surfactant protein A in mouse lung

作　　者：韩芳[1,2] 王丽霞[1,2] 单智焱[1] 王振东[1] 刘春佳[1] 雷蕾[1] 

机构地区：[1]哈尔滨医科大学组织学与胚胎学教研室,黑龙江哈尔滨150081 [2]哈尔滨市第五人民医院,黑龙江哈尔滨150040

出　　处：《解剖科学进展》2012年第5期416-418,共3页Progress of Anatomical Sciences

基　　金：黑龙江省留学归国人员科学技术专项资金资助项目(LC07C17)

摘　　要：目的 探讨异氟醚对小鼠肺表面活性蛋白A表达的影响。方法 将雄性昆明小鼠随机分为5组,吸入空气组、吸入1.0MAC异氟醚5min组、吸入3.0MAC异氟醚5min组、吸入1.0MAC异氟醚2h组、吸入3.0MAC异氟醚2h组;RT-PCR方法检测小鼠肺内SP-A mRNA的表达;免疫组化方法检测小鼠肺内SP-A蛋白的表达。结果 吸入异氟醚5min对小鼠肺内SP-A表达无影响,吸入异氟醚2h能降低小鼠肺内SP-A表达,而且随吸入浓度升高,SP-A表达呈负相关。结论 吸入高浓度异氟醚长时间能够降低小鼠肺内SP-A的表达。Objective To study the effect of isoflurane on the expression of surfactant protein A in mouse lung. Methods Male Kunming mice were randomly divided into 5 groups： air group, 1.0MAC isoflurane for 5min group, 3.0MAC isoflurane for 5min group, 1.0MAC isoflurane for 2h group and 3.0MAC isoflurane for 2h group. The expression of SP-A mRNA and protein in mice lung was examined by RT-PCR and by immunohistoehemistry respectively. Results Inhalation of isoflurane for 5min had no effect on SP-A expression in mouse lung, inhalation of isoflurane for 2h reduced the SP-A expression level in mouse lung dosedependently. Conclusion Inhalation of isoflurane for long time at high concentration inhibits SP-A expression in mouse lung.

关 键 词：异氟醚 肺表面活性蛋白A 小鼠 

分 类 号：H332.2[语言文字—德语]