七氟醚对小鼠肺表面活性蛋白的影响  

Effect of Sevoflurane on expression of surfactant protein in mouse lung

作　　者：王丽霞[1,2] 韩芳[1,2] 单智焱[1] 王振东[1] 刘春佳[1] 雷蕾[1] 

机构地区：[1]哈尔滨医科大学组织学与胚胎学教研室,黑龙江哈尔滨150081 [2]哈尔滨第五人民医院,黑龙江哈尔滨150040

出　　处：《解剖科学进展》2012年第5期452-455,共4页Progress of Anatomical Sciences

基　　金：黑龙江省留学归国人员科学技术专项资金资助项目(LC07C17)

摘　　要：目的 探讨七氟醚对小鼠肺表面活性蛋白的影响。方法 将雄性昆明小鼠随机分为4组,吸入空气组、吸入1.0MAC七氟醚2h组、吸入2.0MAC七氟醚2h组、吸入3.0MAC七氟醚2h组;RT-PCR方法检测了吸入不同浓度七氟醚2h小鼠肺内表面活性蛋白A和B(SP-A、SP-B)mRNA的表达;免疫组化方法检测吸入不同浓度小鼠肺内SP-A、SP-B蛋白的表达。结果 吸入七氟醚2h能降低小鼠肺内SP-A和SP-B表达,而且随吸入浓度升高,SP-A、SP-B表达呈负相关。结论 长时间吸入高浓度七氟醚能够降低小鼠肺内SP-A和SP-B表达。Objective To study the effect of sevoflurane on the expression level of surfactant proteins in the lung of mouse. Methods Kunming male mice were randomly divided into 4 groups： air group, 1.0MAC sevoflurane for 5min group, 2.0MAC sevoflurane for 2h group and 3.0MAC sevoflurane for 2h group. The expressions of SP-A and SP-B mRNA were examined by RT-PCR. SP-A and SP-B proteins expression was detected by imunohistoehemical method. Results Inhalation of sevoflurane reduced SP-A and SP-B expression levels in the lung of mouse dose dependently. Conclusion Inhalation of sevoflurane inhibits SP-A and SP-B expression in mouse lung.

关 键 词：七氟醚 肺表明活性蛋白A 肺表面活性蛋白B 小鼠 

分 类 号：R332.2[医药卫生—人体生理学]