Optimization of a synthetic medium for ethanol production by xylose-fermenting Zymomonas mobilis using response surface methodology  

作　　者：DONG HuiNa ZHAO XueMing MA YuanYuan ZHANG MinHua 

机构地区：[1]Tianjin University R&D Center for Petrochemical Technology,Tianjin University,Tianjin 300072,China [2]Department of Biochemical Engineering,School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China [3]State Key Laboratory of Engines,Tianjin University,Tianjin 300072,China [4]Key Laboratory of Systems Bioengineering,Ministry of Education,Tianjin University,Tianjin 300072,China [5]Edinburgh-Tianjin Joint Research Centre for Systems Biology and Synthetic Biology,Tianjin University,Tianjin 300072,China

出　　处：《Chinese Science Bulletin》2012年第28期3782-3789,共8页

基　　金：supported by the National Basic Research Program of China (2011CBA00804);the National Natural Science Foundation of China(30900033,20806055,20875068);Project of Introducing Talents of Discipline to Universities (B06006)

摘　　要：A synthetic medium for ethanol production by recombinant xylose-fermenting Zymomonas mobilis was optimized using Plackett-Burman(PB) design and response surface methodology(RSM).The effects of 19 medium components were investigated by PB design.Eight of these components were determined to have significant effects on ethanol production.The statistical model was constructed via central composite design(CCD) using five selected variables,including xylose,trisodium citrate,choline chloride,pyridoxine,and thiamine.The validity of the developed model was verified.The ethanol concentration in the optimized medium was 6.7% higher than in the RM medium.The optimized medium was then simplified to give the final synthetic medium(S2),in which the ethanol concentration was 20.7% higher than in the RM medium.Xylose and trisodium citrate were the key medium components influencing ethanol production,while calcium pantothenate was the only growth factor required by recombinant Z.mobilis.Ethanol production and growth response were directly proportional to the logarithm of the concentration of trisodium citrate in the range of 0.1-1.6 g/L.A synthetic medium for ethanol production by recombinant xylose-fermenting Zymomonas mobilis was optimized using Plack- ett-Burman (PB) design and response surface methodology (RSM). The effects of 19 medium components were investigated by PB design. Eight of these components were determined to have significant effects on ethanol production. The statistical model was constructed via central composite design (CCD) using five selected variables, including xylose, trisodium citrate, choline chloride, pyridoxine, and thiamine. The validity of the developed model was verified. The ethanol concentration in the optimized medium was 6.7% higher than in the RM medium. The optimized medium was then simplified to give the final synthetic medium ($2), in which the ethanol concentration was 20.7% higher than in the RM medium. Xylose and trisodium citrate were the key medium components influencing ethanol production, while calcium pantothenate was the only growth factor required by recom- binant Z mobilis. Ethanol production and growth response were directly proportional to the logarithm of the concentration of trisodium citrate in the range of 0.1-1.6 g/L.

关 键 词：合成培养基 乙醇生产 优化重组 发酵单胞菌 木糖发酵 响应曲面法 中心复合设计 柠檬酸三钠 

分 类 号：TQ223.122[化学工程—有机化工]