Identification and gene mapping of a narrow and upper-albino leaf mutant in rice(Oryza sativa L.)  

作　　者：WANG Feng TANG YanQiang MIAO RunLong XU FangFang LIN TingTing HE GuangHua SANG XianChun 

机构地区：[1]Rice Research Institute,Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops,Engineering Research Center of South Upland Agriculture,Ministry of Education,Southwest University,Chongqing 400716,China

出　　处：《Chinese Science Bulletin》2012年第28期3798-3803,共6页

基　　金：supported by the National Natural Science Foundation of China (31171178);the Fundamental Research Funds for the Central Universities (XDJK2009B019)

摘　　要：The leaf blade consists of color and shape traits.Studies of leaf-blade development are important for improvement of rice yield and quality because it is an essential organ for photosynthesis.A narrow and upper-albino leaf mutant(nul1) was identified from among progeny of the indica restorer line Jinhui10 raised from seeds treated with ethyl methane sulfonate.Under field conditions,the mutant displayed narrow and upper-albino leaf blades with significantly decreased photosynthetic pigment contents throughout their development.The narrow-leaf trait is caused by a decreased number of small veins.In contrast to the wild type,the growth period was extended by approximately 8 d and agronomic traits,such as effective panicle number,percentage seed set and 1000-grain weight,declined significantly in the nul1 mutant.Genetic analysis suggested that the narrow and upper-albino leaf characteristics showed coseparation and were controlled by one recessive gene.The Nul1 gene was mapped onto chromosome 7 between the Indel marker Ind07-1 and the Simple Sequence Repeat marker RM21637.The physical distance between the markers was 75 kb and eight genes were annotated in this region based on the rice Nipponbare genome sequence.These results provide a foundation for cloning and function analysis of Nul1.The leaf blade consists of color and shape traits. Studies of leaf-blade development are important for improvement of rice yield and quality because it is an essential organ for photosynthesis. A narrow and upper-albino leaf mutant （null） was identified from among progeny of the indica restorer line Jinhuil0 raised from seeds treated with ethyl methane sulfonate. Under field conditions, the mutant displayed narrow and upper-albino leaf blades with significantly decreased photosynthetic pigment contents through- out their development. The narrow-leaf trait is caused by a decreased number of small veins. In contrast to the wild type, the growth period was extended by approximately 8 d and agronomic traits, such as effective panicle number, percentage seed set and 1000-grain weight, declined significantly in the null mutant. Genetic analysis suggested that the narrow and upper-albino leaf characteristics showed coseparation and were controlled by one recessive gene. The Null gene was mapped onto chromosome 7 between the Indel marker Ind07-1 and the Simple Sequence Repeat marker RM21637. The physical distance between the markers was 75 kb and eight genes were annotated in this region based on the rice Nipponbare genome sequence. These results provide a foundation for cloning and function analysis of Null.

关 键 词：水稻产量 基因定位 突变体 ORYZA 狭窄 白化 鉴定 甲基磺酸乙酯 

分 类 号：S511[农业科学—作物学] S351.52