恶性疟疾多价疫苗的构建及表达  

Construction and expression of a multivalent vaccine against Plasmodium falciparum malaria

作　　者：郭鑫[1] 潘卫庆[1] 张冬梅[1] 

机构地区：[1]第二军医大学病原生物学教研室,上海200433

出　　处：《中国热带医学》2012年第8期913-915,共3页China Tropical Medicine

摘　　要：目的构建恶性疟原虫多表位融合抗原基因(PfCP2E9),并在毕氏酵母中进行高效分泌表达。方法选取本实验室前期构建的融合蛋白PfCP-2.9与疟原虫红内期疫苗候选抗原EBA175IIF2,按一定的次序拼接成该融合基因,并克隆至酵母表达载体,用电转化方法将拼接基因导入毕氏酵母中进行分泌表达。结果拼接的基因在毕氏酵母中高水平分泌表达。结论构建的恶性疟原虫多表位融合抗原基因能在毕氏酵母中高水平分泌表达,为探讨其免疫学功能及作为多价联合疫苗的成分提供了基础。Objective To construct a multi-epitope antigen of Plasmodium falciparum （designated as PfCP2E9）and express in Pichia pastoris as secreted form. Methods PfCP-2.9 and EBA175IIF2 fragment were selected and jointed in tandem to generate the chimeric gene. The expressing plasmid containing the chimeric gene was introduced into Pichia pastoris by electroporation for inducible expression. Results PfCP2E9 gene was expressed at high level in Pichia pastoriz. Conclusion The chimeric PfCP2E9 gene is expressed in Pichia pastoris. The recombinant PfCP2E9 protein provides a base for investigating its immune function and potential as a component of combined malaria vaccine.

关 键 词：恶性疟原虫 红细胞结合抗原 融合抗原 毕氏酵母 疟疾疫苗 

分 类 号：R531.3[医药卫生—内科学]