肿瘤坏死因子-α抑制剂高通量药物筛选模型的建立及筛选药物的体外实验  

Tumor necrosis factor-α inhibitor high-throughput drug screening model for drug screening in vitro experiments

作　　者：刘文斌[1] 周宁[2] 杨秀敏[1] 杨秀颖[3] 王月华[3] 杜冠华[3] 

机构地区：[1]100730北京,首都医科大学附属同仁医院皮肤性病科 [2]中国康复研究中心北京博爱医院 [3]中国医学科学院药物研究所

出　　处：《中国急救复苏与灾害医学杂志》2012年第9期826-828,共3页China Journal of Emergency Resuscitation and Disaster Medicine

基　　金：国家863重点专项基金项目（2004AA223782）

摘　　要：目的建立TNF-α抑制剂的高通量药物筛选模型。方法用对TNF-α敏感的L929细胞株通过MTF法建立TNF-α抑制剂的筛选模型；筛选了样品并对筛得的活性样品进行进一步的评价。结果建直TNF-α抑制剂L929细胞株筛选模型；初筛样品数：870个，复筛样品数：47个，活性药物：8个；对其中的鹿衔草做了淋巴细胞转化抑制实验。结论TNF-α抑制剂的L929细胞株筛选模型经济稳定可以用于高通量药物筛选；鹿衔草可能具有抑制TNF-α作用和一定的免疫调节作用。Objective To establish a high-throughput drug screening model of TNF-α inhibitors. Methods A screening model for inhibitors of TNF-α was established by adopting TNF-α-sensitive L929 cell line with MTT assay. The active samples were further analyzed and evaluated. Results TNF-α inhibitor L929 cell line screening model was utilized for primarily screening of 870 samples, re-screened 47 samles and 8 active drugs, as well as the experiment of Chinese Pyrola herb transformation inhibition assay. Conclusion TNF-α inhibitors L929 cell line screening model with its feature of the low cost and being stable is recommended for high-throughput drug screening. Chinese Pyrola herb may inhibit TNF-α role and carry certain immunomodulatory effect.

关 键 词：TNF-Α 细胞模型 鹿衔草 

分 类 号：R246.5[医药卫生—针灸推拿学]