新生大鼠持续缺氧条件下水通道蛋白4与脑水肿的关系  

The relationship of AQP4 and brain edema under the condition of continue hypoxia in newborn rat

作　　者：贾佳[1] 余加林[1] 孙善全[2] 

机构地区：[1]重庆医科大学附属儿童医院新生儿科/儿童发育疾病研究省部共建教育部重点实验室/儿科学重庆市重点实验室/重庆市儿童发育重大疾病诊治与预防国际科技合作基地,400014 [2]重庆医科大学神经科学研究中心,400016

出　　处：《重庆医学》2012年第28期2959-2961,2963,共4页Chongqing medicine

摘　　要：目的探讨水通道蛋白4(AQP4)在新生大鼠持续缺氧过程中与脑水肿的关系。方法健康10d龄SD大鼠,随机分为对照组和实验组。实验组结扎右侧颈总动脉,然后缺氧不同时间分为缺氧缺血(HI)2h组、HI 4h组、HI 8h组、HI 16h组4个亚组,对照组行假手术。观察每组动物神经行为学改变,各组实验取脑组织,行苏木精伊红(HE)染色、免疫组织化学染色、荧光定量PCR,观察新生大鼠海马CA1区形态变化和AQP4表达水平。结果实验组均出现不同程度的缺氧症状;实验组与对照组比较,有不同程度的水肿,且随着时间的延长,水肿加重,神经元呈现不可逆损伤;AQP4蛋白和RNA的表达水平呈下降趋势。结论 AQP4表达水平下降参与新生大鼠持续缺氧缺血条件下脑水肿的形成。Objective To determine the relationship of AQP4 and brain edema under the condition of continue hypoxia in new- born rat. Methods Healthy and 10-day-old SD rats were randomly divided into control and experimental groups. Experimental group,the right common carotid artery ligation and hypoxia at different times into 2 h, 4 h,8 h, 16 h four sub-groups,the control group were sham-operated. Observed each animal nervous behavior change,and killed them at corresponding time points,hematoxy- lin-eosin staining to observe rat hippocampal CA1 region morphology,and calculated the relative expression of AQP4 by immuno- histochemistry and fluorescence quantitative PCR. Results The experimental group showed different degrees of hypoxia symptoms; the experimental groups, compared with control group,had different degree of edema, and the degree of edema was serious with the extension of time,neurons exhibited irreversible damage;the expression of the AQP4 protein and AQP4 RNA were related down- ward trend. Conclusion The reduced expression of AQP4 participates the formation of cerebral edema in the neonatal rat hypoxic- ischemic conditions.

关 键 词：水通道蛋白4 新生大鼠 缺氧缺血 脑 脑 水肿 

分 类 号：R722.1[医药卫生—儿科]