新生鼠脑缺氧缺血损伤时血红素加氧酶与细胞红蛋白表达的关系研究  

Relation ship between heme oxygenase and cytoglobin expression on the hypoxia-ischemic brain damage in neonatal rat

作　　者：肖丹萍[1] 史雪川[1] 杨汉华[1] 

机构地区：[1]汕头大学医学院第二附属医院儿科,广东汕头515000

出　　处：《中国儿童保健杂志》2012年第9期810-813,共4页Chinese Journal of Child Health Care

基　　金：广东省科技计划项目(2008B680701036);广东省科技计划项目(B2009180)

摘　　要：【目的】探讨血红素加氧酶(heme oxygenase,HO)与细胞红蛋白(cytoglobin,CYGB)表达的关系,为缺氧缺血性脑损伤(hypoxic-ischemic brain damage,HIBD)的防治提供实验依据。【方法】将7日龄新生SD大鼠170只随机分为正常对照组、假手术组、HIBD组、HIBD+Hemin组、HIBD+ZnPP组5组。其中HIBD组、HIBD+Hemin组及HIBD+ZnPP组在HIBD模型制备前12h,分别腹腔注射生理盐水、Hemin及ZnPP,然后制备HIBD模型。术后于0、24、48h采用免疫组化染色法分析HO-1及CYGB蛋白表达特点。【结果】缺氧后HO-1及CYGB表达随时间增长而递增,而且对于同一时间点HIBD+Hemin组HO-1及CYGB的表达均明显高于HIBD+ZnPP组,HIBD组居中,各组间比较差异有统计学意义;5组海马HO-1及CYGB表达均高度相关,0h的r为0.687～0.916、24h的r为0.473～0.903、48h的r为0.659～0.962,皮质两者的表达亦有相同结果。【结论】Hemin诱导HO-1能使CYGB高表达,而ZnPP抑制HO-1则使CYGB表达减少;HO-1及CYGB的表达在HIBD时存在极明显的正相关性;实验证明脑缺氧缺血损伤时HO-1对CYGB的表达可能具有一定的正性调控作用。[Objective] To discuss the relation between the protein expression of heme oxygenase-l（HO-1） and cytoglobin （CYGB） ,and whether hemin would reduce the brain damage against hypoxic-ischemic damage. [Methods] SD rats of 7-day old were divided into 5 groups randomly,including control group, sham-operated group, HIBD group, HIBD ＋ Hemin group,and H IBD＋ ZnPP group. The rats of HIBD＋ Heroin group, HIBD group and HIBD＋ ZnPP group were given Hemin,normal saline or ZnPP 12 hours before the operation. The rats of three last groups were made for HIBD model. All the groups were killed at 0 h,24 h,48 h after HIBD. At 0 h,24 h,48 h after hypoxic-ischemic,observed the protein expression of HO-1 and CYGB in the brain. [Results] The protein expression of HO-1 and CYGB in all HIBD model groups were increased as time gone on. At any time after hypoxic-ischemic brain clamage the expression of HO-1 and CYGB in the HIBD model groups were more than those in sham-operated group and control group. And in three HIBD model groups the expression of HO-1 and CYGB were showed by immunohistochemistry： HIBD＋ Hemin group〉 HIBD group〉 HIBD＋ ZnPP group, comparison of the three groups was with significant difference. The expression of HO-1 and CYGB had positive correlations in contex and hipppcampi in five groups. The relative factors r at Oh after HIBD were 0. 687-0. 916,24 h after HIBD were 0. 473-0. 903,48 h after were 0. 659-0. 962. The contex also had similar result. [Conclusions] Hemin which was the abduction of HO-1 could increase the expression of HO-1 in contex and hippocampus of HIBD＋ Hemin group. ZnPP which was the depressor of HO could decrease the expression of HO-1 of the HIBD＋ZnPP group. The expression of HO-1 and CYGB was consistency, and they may have clear positive correlation.

关 键 词：细胞红蛋白 血红素加氧酶 缺氧缺血性脑损伤 表达关系 

分 类 号：Q95-33[生物学—动物学]