TCR基因修饰的细胞毒性T淋巴细胞构建与应用  

Construction of TCR gene modified cytotoxic T lymphocytes and its application

作　　者：马素娟 史央 时宏珍 

机构地区：[1]南京得康生物技术有限公司南京市免疫细胞生物工程技术研究中心,210019

出　　处：《国际肿瘤学杂志》2012年第9期650-654,共5页Journal of International Oncology

基　　金：江苏省科技厅国际合作项目（BZ2010044）;南京市科委国际合作项目（201101072）;南京市科委高新处重大产业化项目（201103058）

摘　　要：细胞毒性T淋巴细胞（CTL）是机体抗肿瘤和抗病毒感染的关键效应细胞，CTL通过T细胞受体（TCR）特异识别靶细胞表面MHC-I与抗原多肽形成的复合物，进而释放穿孔素、颗粒酶等生物活性物质对靶细胞进行溶解。鉴于CTL在治疗肿瘤和病毒性疾病中的潜在作用，对CTL的基础与应用研究备受关注，大多研究集中于对TCR基因的改造。随着分子生物学的发展，TCR基因克隆、转导技术已较成熟，研究方向主要是如何保证TCR基因高效、正确表达组装成为具生物活性的分子。Cytotoxic T lymphocytes （CTLs） are critical effectors which play important roles both in anti-tumor and anti-virus immune responses. Through T cell receptors （TCRs） , CTLs can specifically recognize MHC- I -peptides complexes presented on the surface of target cells, and then release biological substances such as perforin and granzymes into the target cells and dissolve them. Since the significant potential value of CTLs, more and more people are focusing on its basic and appliedstudies. Most researches are about the transformation of the TCR gene. With the development of molecular biology, cloning and transduction of TCR gene have been more mature. Researchers are finding ways to ensure that TCR gene expresses efficiently and assemble correctly.

关 键 词：细胞毒性T淋巴细胞 基因 T细胞受体 免疫疗法 

分 类 号：R392[医药卫生—免疫学]