日粮组合效应对瘤胃可利用粗蛋白的影响  

Effect of associated effects for ruminant feeds on the utiliable crude protein

作　　者：郭冬生[1] 龚群辉 彭小兰[2,3] 

机构地区：[1]湖南文理学院生命科学院,动物学湖南省高校重点实验室,湖南常德415000 [2]湖南省畜牧兽医研究所,湖南长沙410131 [3]湖南省常德市武陵区畜牧兽医水产局,湖南常德415000

出　　处：《畜牧与兽医》2012年第9期8-13,共6页Animal Husbandry & Veterinary Medicine

基　　金：湖南省自然科学基金项目(12JJ9007);湖南省教育厅优秀青年项目(11B088);湖南省科技成果推广计划项目(2011CK3020);常德市科技局研究项目(2011CK10);“动物学”湖南省“十二五”重点建设学科(湘教发【2011】76)

摘　　要：为了研究日粮组合效应对瘤胃可利用粗蛋白(uCP)的影响,以装有永久性瘤胃瘘管的黄牛作为瘤胃液的供体动物,应用短期人工瘤胃发酵技术研究日粮组合效应对瘤胃可利用粗蛋白(uCP)和瘤胃NH3-N的影响。结果表明,混合日粮按照单一饲料样品的uCP加权求和值(x)与实际测定的uCP(y)之间存在显著的一元回归关系(y=1.06x-2.67,R2=0.82,P<0.01,n=24)。混合日粮按照加权求和的uCP(x)和实际测定的uCP(y)值之间差异极显著(t检验,P<0.01),表明对uCP而言,饲料间在瘤胃内存在极显著的日粮组合效应。研究还发现,NH3-N和uCP发生相反类型的组合效应,这是因为瘤胃微生物利用饲料分解产生的NH3-N合成微生物蛋白(MCP),造成瘤胃中NH3-N浓度下降,但合成MCP会使uCP升高。The utilizable crude protein （uCP） includes the rumen microbial crude protein （MCP） and the rumen un-degradable dietary crude protein （UDP）. In this study, one adult cattle fitted with a perpetual large rumen cannula was used as the donor of rumen fluid. The animal was fed with a mixed ration （33% concentrate） twice daily and had free access to water. The results showed that there was a signifi- cant regression relationship between the uCP value calculated by associated effects （x） and those measured by the in vitro incubation tech- nique （y, 24 h incubation） ： y = 1.06x-2. 67 R2 = 0. 82 P〈0. 01 n = 24. The uCP value had significant associated effects （ t tested be- tween x and y, P=0. 002〈0. 01 ）. In addition, NH3-N and uCP were the opposite types of associated effects because the MCP was synthe- sized to make use of the NH3-N in the rumen.

关 键 词：人工瘤胃 组合效应 可利用粗蛋白 氨态氮 

分 类 号：S823.9[农业科学—畜牧学]