丙泊酚预处理对单肺机械通气大鼠肺组织血红素氧合酶-1表达的影响  

Effect of preconditioning with propofol on expression of HO-1 protein in rat lung tissue during one lung ventilation

作　　者：巴玉峰[1] 冯惠民[2] 李长生[2] 李印[1] 程金华[1] 邢文群[1] 刘宝兴[1] 

机构地区：[1]河南省肿瘤医院胸外科,郑州450003 [2]河南省肿瘤医院麻醉科,郑州450003

出　　处：《郑州大学学报（医学版）》2012年第4期500-502,共3页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:研究丙泊酚预处理对单肺机械通气(OLV)时大鼠肺组织血红素氧合酶-1(HO-1)表达的影响。方法:36只雄性SD大鼠随机分成6组(n=6):对照(C)组,双肺通气(T)组,右侧单肺通气-左肺(OL)组,右侧单肺通气-右肺(OR)组,丙泊酚-右侧单肺通气-左肺(POL)组,丙泊酚-右侧单肺通气-右肺(POR)组。T组插气管导管双肺通气;OL、OR、POL和POR组大鼠麻醉后行右侧单肺通气,POL和POR组大鼠单肺通气1h前静脉泵入丙泊酚(50mg/kg)。C组大鼠麻醉后,直接开胸取左全肺。T、OL和POL组大鼠通气1h后取出左全肺,OR和POR组大鼠取出右全肺,行湿/干质量(W/D)比值计算,并采用Westernblot法测定HO-1蛋白的表达。结果:各组大鼠肺组织W/D比较差异有统计学意义(F=65.371,P<0.001),OL、OR组大鼠肺组织W/D高于C组(P<0.05)。各组大鼠肺组织HO-1蛋白表达差异有统计学意义(F=72.851,P<0.001),T、OL、OR组大鼠肺组织中HO-1蛋白的表达高于C组,POL组高于OL组,POR组高于OR组(P<0.05)。结论:丙泊酚预处理可以使大鼠肺组织HO-1表达增加,减轻OLV所造成的组织水肿。Aim ：To investigate the effect of preconditioning with propofol on the expression of heme oxygenase-1 （ HO- 1 ） protein in rat lung tissue during one lung ventilation（OLV）. Methods：Thirty-six male SD rats were randomly divided into 6 groups（6 rats in each group） ： group C as control; group T, two lung ventilation;and group OL, OR,POL and POR, with right-OLV. Rats in group POL and POR was anesthetized by inhalation of propofol （50 mg/kg） 1 h before OLV. The left lung tissue samples in group C,T, OL, POL and the right lung tissue samples in group OR, POR were collected to weigh and calculate the ratio of wet/dry（W/D） , and to measure the expression of HO-1 protein by Western blot. Flesults：W/D a- mong the 6 groups were obviously different（ F = 65. 371, P 〈 0. 001 ）. W/D in group OL, OR was significantly higher than that in group C （P 〈 0.05 ）. The expression of HO-1 in lung tissue among the above 6 groups were obviously different（ F =72. 851 ,P 〈 0. 001 ）. Compared with group C, the expression of HO-1 in group T, OL, OR was dramatically increased;the expression of HO-1 in group POL was higher compared with group OL. Furthermore,there was a remarkable increasing ex- pression of HO-1 in group POR than that in group OR（ P 〈 0.05 ）. Conclusion ： Preconditioning with propofol can up-regu- late the expression of HO-1 and relieve pulmonary edema induced by OLV.

关 键 词：丙泊酚 单肺机械通气 血红素氧合酶-1 大鼠 

分 类 号：R655.3[医药卫生—外科学]