替米沙坦对大鼠心肌肥厚组织中RASSF1A表达的影响  

Effect of telmisartan on RASSF1A expression in isoproterenol-induced myocardial hypertrophy tissue of rats

作　　者：王伯乐[1] 赵玉兰[1] 张强[1] 董静[1] 

机构地区：[1]郑州大学第二附属医院心内科,郑州450014

出　　处：《郑州大学学报（医学版）》2012年第4期521-524,共4页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:观察替米沙坦对心肌肥厚大鼠左室心肌组织中RASSF1A表达的影响。方法:SD大鼠30只,随机分为对照组、模型组和干预组。模型组和干预组给予异丙肾上腺素3mg/(kg·d)皮下注射,干预组在建模同时灌胃替米沙坦8.33mg/(kg·d)。10d后,进行血流动力学和心肌肥厚指数测定;取大鼠左心室心肌组织,分别采用RT-PCR和免疫组化法检测RASSF1AmRNA和蛋白的表达,同时观察病理学变化。结果:血流动力学、心室肥厚指数及病理观察结果提示,模型组大鼠发生心肌肥厚,干预组上述变化较模型组减轻。3组大鼠心肌组织中RASSF1AmRNA和蛋白的表达差异均有统计学意义(F=38.968,284.445,P<0.001)。与对照组相比,模型组、干预组RASSF1AmRNA和蛋白的表达降低(P<0.05);与模型组相比,干预组RASSF1AmRNA和蛋白的表达升高(P<0.05)。结论:替米沙坦可通过调节RASSF1A的表达抑制心肌肥厚的发生。Aim：To investigate the effect of telmisartan on expression of RASSF1A in hypertrophy myocardial tissue of rats induced by isoproterenol.Methods：Thirty rats were randomly divided into three groups and 10 rats in each group：control group,model group（3 mg·kg-1·d-1 isoproterenol for 10 d） and intervention group（3 mg·kg-1·d-1 isoproterenol and 8.33 mg·kg-1·d-1 telmisartan for 10 d）.RASSF1A mRNA and protein in left ventricular tissues were analyzed by RT-PCR and immunohistochemistry,respectively.Results：The expressions of RASSF1A mRNA and protein in the 3 groups were statistically different（F = 38.968,284.445,respectively,P 〈 0.001）.The above indexes in the model group and intervention group were lower than those of the control group（P 〈 0.05）,but those of the intervention group were higher than the model group（P 〈 0.05）.Conclusion：Telmisartan can inhibit myocardial hypertrophy induced by isoproterenol throughregulating the expression of RASSF1A.

关 键 词：RASSF1A 心肌肥厚 替米沙坦 大鼠 

分 类 号：R542.2[医药卫生—心血管疾病]