铜绿假单胞菌6型O-特异性多糖与破伤风类毒素结合物免疫原性研究  

Study on the immunogenicity of a conjugate composed of Pseudomonas aeruginosa(IATS 6)O-specific polysaccharide bound with tetanus toxoid

作　　者：谢茂超 陈明拓 姜晓 杨硕 王学林[1] 葛永红[1] 

机构地区：[1]成都生物制品研究所有限责任公司,成都610063 [2]成都蓉生药业有限责任公司,成都610041

出　　处：《微生物学免疫学进展》2012年第4期10-14,共5页Progress In Microbiology and Immunology

摘　　要：目的研制有效防治铜绿假单胞菌感染的疫苗。方法用热酚水法提取铜绿假单胞菌6型(IATS 6)菌株的脂多糖(LPS),去除类脂A并纯化O-特异性多糖(O-SP),用CDAP活化O-SP,己二酸二肼作连接臂,在EDAC作用下,与破伤风类毒素结合制备出O-SP-TT结合物并对该结合物进行小鼠免疫原性试验和免疫保护性试验。结果制备的结合物在小鼠免疫原性试验中,产生了高效价的IgG抗体,与O-SP试验组相比,差异具有统计学意义(P<0.05);免疫保护性试验表明,结合物免疫小鼠能很好的保护5～10 LD50活菌的腹腔攻击。结论该结合物有望成为防治IATS 6型铜绿假单胞菌感染的有效疫苗。Objective To develop an O-specific polysaccharide （O-SP） based conjugate against Pseudomonas aeruginosa. Methods Lipopolysaccharide（LPS） from Pseudomonas aeruginosa （IATS 6 ） was isolated via hot phenol extraction and then hydrolyzed to obtain O-specific polysaccharide （ O-SP ）. O-SP was activated with CDAP,using adipic acid dihydrazide as a linker. Activated O-SP was conjugated to tetanus toxoid via EDAC mediated condensation reaction. Immunogenicity of the conjugate and its immune protection were evaluated in mice. The challenge test was conducted in mice immunized with the conjugate. Results Significantly high IgG anti-IATS 60-SP levels were induced in the mice immunized with the con-jugates comparing with those of immunized with O-SP （ P〈0.05 ）. The potency study showed that the mice immunized with the conjugate survived when challenged with bacteria of 5--0 LD50. Conclusion The vaccine is expected to effectively a-gainst IATS 6 Pseudomonas aeruginosa.

关 键 词：铜绿假单胞菌 O-特异性多糖 结合疫苗 免疫原性 免疫保护性 

分 类 号：R392[医药卫生—免疫学]