Coγ射线累积照射对大鼠尿液中小分子代谢物的影响  

Effects of accumulated 60 Co y-ray irradiation on the small molecular metabolites in rats urine

作　　者：张慧芳[1] 杨彪[1] 党旭红[1] 刘建功[1] 邢利虹[1] 郭月凤[1] 左雅慧[1] 段志凯[1] 刘占旗[1] 

机构地区：[1]中国辐射防护研究院,太原030006

出　　处：《中华放射医学与防护杂志》2012年第4期358-362,共5页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的探讨60Co．Y射线累积照射对大鼠尿液中小分子代谢物的影响。方法健康成年雄性sD大鼠10只，用60Coy射线照射大鼠，累积剂量分别为0、0．2、0．4、0．6、0．8和1．0Gy，连续照射5d。采用核磁共振（1HNMR）技术，结合主成分分析法（PCA）和偏最小二乘法判别（PLS—DA），分析各累积剂量下大鼠尿液中小分子代谢物的变化。结果大鼠尿液中的小分子代谢物发生了明显的改变，与对照组相比，0．2—1．0Gy各组乙酸乙酯的相对含量显著降低（t=29．7—30．7，P〈0．05），且其相对含量随剂量增加基本稳定（P〉0．05）；马尿酸的相对含量增加（t=4．4～21．6，P〈0．05），且当累积剂量达到1Gy时其相对含量显著增加（t=21．6，P〈0．05）。三甲胺氮氧化物、牛磺酸、脯氨酸相对含量均比对照组增加（t：3．5～13．4、4．7—11．5和2．9～12．7，P〈0．05），且这3种代谢物的相对含量随剂量增加有相同的变化趋势。结论60Co．Y射线累积照射后，尿液中乙酸乙酯、马尿酸、三甲胺氮氧化物、牛磺酸、脯氨酸有可能成为累积照射的辐射损伤标志物。Objective To explore the effects of accumulated 60Co.Y-ray irradiation on small molecular metabolites in rats urine. Methods Ten healthy male SD rats were irradiated by 60Co.Y-rays in 5 days and the accumulated doses were 0, 0. 2, 0. 4, 0. 6, 0. 8, 1.0 Gy, respectively. The metabolites in urine of different groups were measured with I H-NMR combined with principal components analysis （PCA） and partial least square discriminate analysis （PLS-DA）. Results The metabolites in rat urine were obviously changed after irradiation. Compared with control group, the amount of acetoaeetate decreased after irradiation（t = 29. 7 -30. 7,P〈 0. 05）, but its relative level was stable when the dose increased （ P 〉 0.05 ）. Meanwhile, the relative level of hippuric acid increased（ t = 4.4 - 21.6 ,P 〈 0. 05 ） especially when the accumulated dose was higher than 1 Gy （t = 21.6, P 〈 0.05 ）. The relative level of proline, taurine and trimethylamine-N-oxide increased after irradiation with the same trend （t = 3.5 - 13.4,4. 7 - 11.5,2.9- 12.7,P〈0.05）. Conclusions The acetoaeetate, hippurie acid, proline, taurine, and trimethylamine-N-oxide may be applicable for biomarkers of accumulative irradiation on rat.

关 键 词：累积照射 尿液代谢成分 核磁共振 主成分分析 

分 类 号：R142[医药卫生—公共卫生与预防医学]