Toll样受体2介导牙龈成纤维细胞中白细胞介素6家族细胞因子的表达  

Expression of cytokines of interleukin-6 family in gingival fibroblasts by Toll-like receptor-2

作　　者：辛红[1] 王艳华[1] 刘浩[1] 

机构地区：[1]天津市人民医院口腔科,300121

出　　处：《中华口腔医学杂志》2012年第9期523-527,共5页Chinese Journal of Stomatology

摘　　要：目的在牙龈成纤维细胞中观察能否通过To11样受体2（Toll．1ikereceptor-2，TLR-2）信号通路影响一些细胞因子的表达，以期探讨牙周炎的发病机制。方法将体外培养的人正常牙龈成纤维细胞分为空白对照组、大肠杆菌Bckri如施col（Ec）脂多糖组及牙龈卟啉单胞菌（Porphyromonas gingivalis，Pg）脂多糖组，应用实时荧光定量聚合酶链反应技术观察白细胞介素6（intedeukin-6，IL-6）家族成员IL-6、IL-11、白血病抑制因子（leukemia inhibitoryfactor，IJIF）及抑瘤素M等细胞因子基因的表达情况，酶联免疫吸附测定法分析蛋白的表达。对各组间的mRNA和蛋白表达的比较采用单因素方差分析，检验水准为单侧α=0．05。结果Pg中的脂多糖可以刺激IL-6和uF的mRNA和蛋白表达，与空白对照相比10hIL-6mRNA的表达水平最高（5．87±0．83），随着Pg的浓度升高表达量也增加，IL-11或抑瘤素MmRNA表达则不受影响。Pg脂多糖处理48h后，IL-6和LIF蛋白的表达显著增强，酶联免疫吸附测定检测结果分别为（962±57）ng／L和（47±18）ng/L。用特异性TLR-2激动剂处理后发现在牙龈成纤维细胞中TLR-2对IL-6和LIF的产生发挥了重要作用。结论在人牙龈成纤维细胞中通过TLR-2信号通路可以控制IL-6细胞因子的表达。Objective To investigate whether signaling through Toll-like receptor-2 （TLR-2） can affect the expression of some cytokines in human gingival fibroblasts. Methods The gingival fibroblasts were isolated and cultured in vivo, divided into blank control group, lipopolysaccharide （LPS） from Porphyromonas gingivalis（Pg） group and Escherichia coli（Ec） group, mRNA expression levels were measured by real-time polymerase chain reaction （PCR）. The protein expression levels were detected by the enzyme linked immunosorbent assay（ELISA）. The data was statistically analyzed by SPSS16. 0 software package. Results LPS from Pg could stimulate the expression of interleukin（IL）-6 and leukemia inhibitory factor（LIF） mRNA and protein, which reached the peak （5.87 -＋ 0. 83 ） at 10 h, and the expression level increased with the increase of the Pg concentration. IL-11 or oncostatin-M （OSM） mRNA expression was not affected by LPS. After treated with Pg for 48 h, the protein expression of IL-6 and LIF was up-regnlated, （ 962 ±57 ） ng/L and （47 ± 18 ） ng/L respectively. Conclusions Signaling through TLR-2 controls the expression of cytokines of IL-6 family in human gingival fibroblasts.

关 键 词：成纤维细胞 白细胞介素6 白血病抑制因子 TOLL样受体2 

分 类 号：R285.5[医药卫生—中药学]