4-羟苯维胺酯抑制大鼠膀胱癌的COX-2的表达和诱导肿瘤细胞凋亡的实验研究  

Prevention effect of 4-HPR on bladder tumor in SD rats:Anin vivo experiment

作　　者：沈群山[1] 黄后宝[1] 潘彩明[1] 薛珺[1] 蔡张愉[1] 王啸[1] 姜书传[1] 

机构地区：[1]皖南医学院弋矶山医院泌尿外科

出　　处：《中国临床药理学与治疗学》2012年第9期972-977,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：安徽省教育厅临床医学应用技术科研课题(2006KJ136C)

摘　　要：目的:使用N-甲基-N-亚硝基脲(N-methyl-N-nitrosourea,MNU)诱导SD大鼠膀胱肿瘤模型,观察4-羟苯维胺酯(4-HPR)的对大鼠膀胱肿瘤的干预效果。方法:70只SD大鼠随机分成对照组(A组)、造模组(B组)、造模处理组(C组、D组、E组),处理组分别予以4-HPR、阿霉素(ADM)、卡介苗(BCG)行膀胱内灌注。给药结束后1周处死大鼠,用免疫组化检测大鼠膀胱肿瘤中COX-2的表达和微血管密度(MVD);用TUNEL法检测凋亡。结果:标本病理结果提示4-HPR、BCG、ADM对MNU诱导肿瘤的发生有明确的干预效果(P<0.05)。4-HPR能有效抑制COX-2的表达及微血管的生成并促使肿瘤细胞的凋亡(P<0.05)。结论:4-HPR、BCG、ADM对MNU的致癌过程均有干预作用,4-HPR与ADM、BCG的抗癌作用无显著性差异,且其副反应更少。AIM： To observe the curative effect of 4-HPR on bladder tumor using bladder tumor model induced by MNU in SD rats. METHODS： 70 SD rats was randomly divided in-to 5 groups： control group （Group A）, module group （Group B）, mould processing group （Group C, Group D, Group E）. The bladder perfusion of treatment group was instilled 4-HPR, ADM and BCG respectively. A week af-ter administration, the SD rats were put to death. The immunohistochemistry was used to detecte the expression of COX-2 and MVD, and the TUNEL technique was applied to examine apoptosis. RESULTS： The results of pathological specimens indicated that the 4-HPR, ADM and BCG inducing tumor had an obvious intervention effect （P〈0.05）; Besides, the 4-HPR could in- hibit the expression of COX-2, the generation of capillaries, and the spur of apoptosis tumor cells （P〈0.05）. CONCLUSION： The 4-HPR, ADM and BCG to MNU had an intervention effect in carcinogenic process. The 4-HPR had no signifi-cant differences in anticancer effect compared with other groups, but had less side effect than the other groups.

关 键 词：膀胱肿瘤 4-羟苯维胺酯 卡介苗 阿霉素 

分 类 号：R965.2[医药卫生—药理学]