槐米中槲皮素提纯、鉴定以及诱导人乳腺癌MCF-7细胞凋亡作用  

Effects of quercetins extracted and isolated from flos sophorae immaturus and apoptosis in MCF-7 human breast cancer cell in vitro

作　　者：张海英 徐水凌[2] 张力衡[2] 

机构地区：[1]仙居县中医院 [2]嘉兴学院医学院

出　　处：《中国临床药理学与治疗学》2012年第9期995-1000,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：浙江省中医药科技计划项目(2008CA108)

摘　　要：目的:研究从槐米中槲皮素(Quercetin,Que)提取、鉴定和对人乳腺癌MCF-7细胞生长的抑制以及诱导凋亡作用。方法:采用碱溶解酸沉淀法提纯槐米中槲皮素,并进行红外光谱分析;采用体外培养人乳腺癌MCF-7细胞,用不同浓度槲皮素处理;采用四甲基偶氮唑蓝(MTT)比色法测定细胞生长抑制率;采用细胞凋亡DNA Lad-der和FITC-Annexin V/PI荧光标记流式细胞仪检测,槲皮素作用后MCF-7细胞凋亡情况。结果:不同浓度槲皮素对人乳腺癌MCF-7细胞有生长抑制作用,并呈浓度和时间依赖性(P<0.05);10.0mmol/L槲皮素作用MCF-7细胞2d,DNA电泳出现特征性的凋亡条带;10.0mmol/L槲皮素处理MCF-7细胞1d、2d和3d,细胞凋亡率分别为(9.2±1.5)%、(30.0±11.8)%和(60.8±10.6)%。结论:用碱溶解酸沉淀法可从槐米中提纯槲皮素,提取的槲皮素对人乳腺癌MCF-7细胞有生长抑制和诱导凋亡作用。ABSTRACT AIM： To investigate the effects of quercetins extracted and isolated from the flos sophorae immaturus and apoptosis in MCF-7 hu-man breast cancer cell. METHODS： With the al- kali-soluble acid precipitation methods, the quercetins were extracted from flos sophorae im- maturus and were identificated by Infrared spec-trophotometry. Colorimetric MTT assay was used to measure the prolification inhibitions of the quercetin in MCF-7 cells. The apoptosis of treated cells were examined by DNA agarose gel electrophoresis analysis of DNA fragmentation and flow cytometry using fluorescein labeling FITC-Annexin V/PI, respectively. RESULTS： MTT results showed that the different doses of quercetin can inhibit the growth of MCF-7 cells in dose-and time-dependent significantly （P〈0.05）. The characteristic bands of apoptosis by DNA electrophoresis were found in MCF-7 cells treated by quercetin at 10.0 mmol/L for 2 d. The rates of apoptosis of MCF-7 cells which were treated with quercetin at 10.0 mmol/L for 1 d, 2 d and 3 d were （9.2±1.5）%, （30.0±11.8）% and （60.8 ± 10.6）%, respectively. CONCLUSION： The quercetins can be purified from the flos sophorae immaturus with the li-soluble acid precip cetin can inhibit MCF-7 cells. itation methods. e growth and a popto alka-quer-sis in

关 键 词：槲皮素 槐米 人乳腺癌MCF-7细胞 诱导凋亡 

分 类 号：R965.2[医药卫生—药理学]